Study on production of soluble dietary fiber from soybean dregs by technology of steam-cooking combining with ultrasonic-assisted enzymatic hydrolysis
The production of soluble dietary fiber from soybean dregs was explored by technology of steam-cooking combining with ultrasonic-assisted enzymatic hydrolysis.In order to further intensify SDF production,the production technical parameters were optimized by orthogonal array design on the basis of a series of single-factor experiments.Experimental results indicated that the optimal high temperature modification condition for pretreated soybean dregs as follows:Liquid-solid ratio 10mL/g,temperature of steam-cooking 130℃,treatment time 100min.And the optimal ultrasonic-assisted enzymatic hydrolysis condition for modified sample as follows:enzyme-substrate ratio 1200:1,liquid-solid ratio 20:1,the power of ultrasonic 650W,treatment time 120min.Under these conditions,about 3.91g SDF could be extracted from 10g pretreated sample.The qualitative characters of modified samples with steam-cooking could be improved by ultrasonic-assisted enzymatic hydrolysis.Under such conditions,the water holding capacity and swelling capacity was 14.48g/g and 12.3mL/g.This experimental conclusion had great significance for production and commercial application of SDF.
soluble dietary fibersoybean dregscellulosehigh temperature modificationultrasonic