Study on the interaction between dihydromyricetin and trypsin
The interaction between dihydromyricetin（DMY） and trypsin was studied by measuring the catalytic activity,the change in the antioxidant activity and fluorescence spectra after being interacted with trypsin, After treatment for 10min at 37~C,dihydromyricetin led to the inhibition of catalytic activity. Trypsin could mask the total antioxidant capacity by scavenging ABTS DMY led the quenching of intrinsic fluorescence of trypsin in physiological condition. With fluorescence quenching method,the quenching constant Kq was found to be 2.3180×10^12（mol/L）-1S-1 and the number of binding site N was 0.6819.
国家科技期刊平台
NSTL
万方数据
维普
