首页|7S-葡聚糖糖基化产物及其水解物制备纳米颗粒的研究

7S-葡聚糖糖基化产物及其水解物制备纳米颗粒的研究

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采用干热法制备β-伴大豆球蛋白(7S)与葡聚糖(67ku)糖基化产物.利用胰蛋白酶对糖基化产物进行限制性酶解,得到了一种具有两亲性结构的水解度(DH)为2.2%的糖基化产物水解物.利用反溶剂法制备了7S、7S-葡聚糖糖基化产物及DH为2.2%的糖基化产物水解物的纳米颗粒,并且考察了戊二醛交联和旋转蒸发去除乙醇对已形成的纳米颗粒的影响.研究了三种纳米颗粒样品的粒度,Zeta-电位和多分散性指数(PDI).实验发现,DH为2.2%的糖基化产物水解物在反溶剂过程中更容易发生自组装,形成的纳米颗粒比7S和7S-葡聚糖糖基化产物形成的纳米颗粒的粒度小、电位绝对值高、PDI值低.
Study on nanoparticles synthesized from β-conglycinin-dextran conjugate products and conjugate products hydrolysates
The amphiphilic molecule was prepared by conjugating soy β-conglycinin and dextran(MW 67ku) under dry-heated reaction followed by trypin hydrolysis with the degree of hydrolysis(DH) at 2.2%. Nanoparticles were synthesized from β-conglycinin , β-conglycinin-dextran conjugate products and hydrolysates of β -conglycinin -dextran conjugate products DH 2.2% by desolvation method. The effect of glutaraldehyde cross-linking and removing ethanol by evaporation on the formed nanoparticles were examined. Formed nanoparticles were systematically investigated by means of particle size,zeta-potential and polydispersity index(PDI). Self-assembly could be easily occurred in the hydrolysates of β-conglycinin-dextran conjugate products DH 2.2% during desolvation process,and the formed nanoparticles had smaller particle size,much higher absolute value of zeta-potential and a significantly lower PDI compared with that of the nanoparticles formed from β-conglycinin,and β-conglycinin-dextran conjugate products.

β-conglycinindextranconjugatenanoparticlesamphiphilic structure

张晋博、王丽娟、何秀婷、杨晓泉、尹寿伟、王金梅

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华南理工大学轻工与食品学院蛋白质工程研究中心,广东广州510640

沈阳师范大学粮食学院,辽宁沈阳110034

7S 葡聚糖 糖基化 纳米颗粒 两亲性结构

国家自然科学基金

21076087

2013

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2013.34(8)
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