Study on nanoparticles synthesized from β-conglycinin-dextran conjugate products and conjugate products hydrolysates
The amphiphilic molecule was prepared by conjugating soy β-conglycinin and dextran(MW 67ku) under dry-heated reaction followed by trypin hydrolysis with the degree of hydrolysis(DH) at 2.2%. Nanoparticles were synthesized from β-conglycinin , β-conglycinin-dextran conjugate products and hydrolysates of β -conglycinin -dextran conjugate products DH 2.2% by desolvation method. The effect of glutaraldehyde cross-linking and removing ethanol by evaporation on the formed nanoparticles were examined. Formed nanoparticles were systematically investigated by means of particle size,zeta-potential and polydispersity index(PDI). Self-assembly could be easily occurred in the hydrolysates of β-conglycinin-dextran conjugate products DH 2.2% during desolvation process,and the formed nanoparticles had smaller particle size,much higher absolute value of zeta-potential and a significantly lower PDI compared with that of the nanoparticles formed from β-conglycinin,and β-conglycinin-dextran conjugate products.