Optimization of Bacillus subtilis fermentation conditions and preparation of its microecological probiotics
This study focused on optimization of Bacillus subtilis fermentation conditions and preparation of its microecological probiotics.With single factor trial and orthogonal experiment,the optimal fermentation liquid media were determined as glucose 1.0％,beef extract 1.5％ and K2HPO4 0.5％.The optimal fermentation process conditions were obtained as the fermenting temperature 30℃,the initial pH 7.2,inoculating quantity 4％ and fermenting time 38～40h.Under the optimal conditions,the maximum live cell concentration reached 3.98 x 109cfu/mL After fermentation,the microecological probiotics was prepared by emulsification and extrusion using 1％ sodium alginate and 4％ CaCl2.The live cell counting method was used to inspect the stability of the microecological probiotics.The stability tests showed that both of the survival rates of the microecological probiotics by emulsification and extrusion were much higher than unmicroencapsulation probiotics.Compared to the extrusion method,the emulsification preparation will be more suitable for large-scale industrial production.
国家科技期刊平台
NSTL
万方数据
维普
