Optimization of the preparation of konjac oligo-glucomannan in semi-drying enzymatic hydrolysis method and its antioxidant capacity
Objective:To optimize the preparation of konjac oligo-glucomannan in semi-drying enzymatic hydrolysis method.Method:Based on one-factor-at-a-time experiments,a three-variable,three-level Box-Behnken experimental design was applied to establish a quadratic polynomial model for the hydrolysis rate of konjac glucomannan as a function of enzymolysis time,beta mannan activity and Konjac Glucomannan-to-buffer solution ratio;and then the antioxidation of its product was determined.Results:the optimal reaction condition were pH6,reaction temperature of 55℃,reaction time of 4.2h,konjac glucomannan-to-buffer solution ratio of 1∶1.63 and beta mannan activity of 1500U/g ;The IC50 to·OH and O2-· of konjac oligo-glucomannan prepared in this method were 0.4331、15.7mg/mL,and its reducing ability was 73.82％ of Vc at the same concentration.Conclusion:the experimental hydrolysis rate of konjac glucomannan obtained under these conditions was 52.67％,which was consistent with the theoretical value,and the optimized result was reliable and could be used in practice; The product had great antioxidant capacity.
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NSTL
万方数据
维普
