Samples were extracted with water by heating reflux and the extract was filtration.The supernatant was then analyzed by NH2 column with elution (acetomitrile-5mmol/L ammonium acetate solution (80∶20) as mobile phase) and quantified by external standard method.A good linearity was shown in the range of 0.005～0.106mg/mL.The average recoveries at three spike levels (0.011,0.014,0.017mg/mL) were 102.0％,100.2％ and 101.5％,respectively.The contents of ergothioneine in mushrooms at three different concentration were in the range of 0.000％～0.280％.The method was simple and reliable,and might be used for the determination of ergothioneine in the mushrooms of different species and the foundation for the application of rare amino acids.
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维普
万方数据
