Research of blanching sasanquasaponin by reduction agents
This study was to decolorize sasanquasaponin (SQS)solution effectively by reduction method.The response surface methodology(RSM) was used to optimize conditions for blanching SQS solution.The following parameters:dosage of blanching agents(KBH4 and NaHSO3),pH of the solution and reaction temperature were the variables.The optimal conditions determined by response surface methodology were:the dosage of KBH4 was 1.2% (w/w),the dosage of NaHSO3 was 2.2% (w/w),pH of 4.8 and reaction temperature of 51.0℃.The △E value under the optimal condition was 31.324 ± 0.378 and the yield of sasanquasaponin was 95.8%.Further detection of LC-MS/MS showed that bleaching agents did not degrade the structure of sasanquasaponin.