首页|川渝地区金银花HPLC指纹图谱的研究

川渝地区金银花HPLC指纹图谱的研究

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采用HPLC法测定十个不同商品来源的金银花样品,建立川渝地区金银花药材甲醇溶解部分的HPLC指纹图谱,为科学评价与有效控制金银花质量提供新方法.色谱条件为C18柱,甲醇-1%磷酸为流动相进行梯度洗脱,检测波长237nm,体积流量1.0mL/min,柱温30℃.结果表明,该方法能够很好地分离川渝地区金银花的主要成分;不同产地金银花药材指纹图谱相似度较好,建立了含有11个特征指纹峰的川渝地区金银花标准指纹图谱;HPLC标准指纹图谱具有良好的重现性和特征性.
HPLC fingerprints of Flos Lonicerae in Sichuan-Chongqing
10 samples of Flos Lonicerae were tested by HPLC,which were from sichuan-chongqing.HPLC fingerprint of the methanol-dissolved elements provided a new way for scientific evaluation and effective quality control of Flos Lonicerae.The HPLC condition was that:Kromasil TM C18column,washing solution of methanol-1% phosphoric acid,237nm wave length,1.0mL/min flow rate,30℃ column temperature.The result showed that:the common model of HPLC fingerprint was made and the similarity of Flos Lonicerae from different areas was compared,the various ingredients could be well separated from Sichuan-Chongqing Flos Lonicerae by this way,and the standard fingerprint was established with 11 characteristic fingerprint peaks.The HPLC fingerprint had a good similarity,reproducibility and characteristic.This study would be good for the standard planting and quality control of Flos Lonicerae in Sichuan-Chongqing region.

Flos Loniceraechlorogenic acidHPLCfingerprintquality control

赵君峰、马丽苹、白志川

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河南科技大学食品与生物工程学院,河南洛阳471023

西南大学园艺园林学院,重庆北碚400715

金银花 绿原酸 高效液相色谱法 指纹图谱 质量控制

2014

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2014.35(7)
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