HPLC fingerprints of Flos Lonicerae in Sichuan-Chongqing
10 samples of Flos Lonicerae were tested by HPLC,which were from sichuan-chongqing.HPLC fingerprint of the methanol-dissolved elements provided a new way for scientific evaluation and effective quality control of Flos Lonicerae.The HPLC condition was that:Kromasil TM C18column,washing solution of methanol-1% phosphoric acid,237nm wave length,1.0mL/min flow rate,30℃ column temperature.The result showed that:the common model of HPLC fingerprint was made and the similarity of Flos Lonicerae from different areas was compared,the various ingredients could be well separated from Sichuan-Chongqing Flos Lonicerae by this way,and the standard fingerprint was established with 11 characteristic fingerprint peaks.The HPLC fingerprint had a good similarity,reproducibility and characteristic.This study would be good for the standard planting and quality control of Flos Lonicerae in Sichuan-Chongqing region.
Flos Loniceraechlorogenic acidHPLCfingerprintquality control