HPLC-ESI-MS analysis of the structure and content of anthocyanins in purple pakchoi
Contents and structure of anthocyanins in purple pakchoi were identified by high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS).Two kinds of anthocyanins were found in "New ZIGUAN" purple pakchoi.The mainly existence of anthocyanins were acylation form,there were 10 anthocyanins and 9 isomeric compounds.The total anthocyanin content of purple pakchoi was 51.36μg/g·fw,which was calculated as the reference of cyanin-3,5-diglucoside standard.Among them,cyanidin content was 50.71μg/g·fw,accounting for 98.73%.And delphinidin content was 0.65μg/g·fw,accounting for 1.27%.Cyanidin-3-feruloyl-sophoroside-5-malonyl-glucosidase was the major anthocyanin,accounting for 31.85% (16.36μg/g·fw) of the total anthocyanins.