Optimization of protease hydrolysis conditions on silkworm pupa protein
Purpose:Enzyme hydrolysis of silkworm pupa protein was studied in order to provide references to deeply exploit silkworm pupa protein.Methods:Protease was used to hydrolyze silkworm pupa protein and spectrophotometry was used to detect indicators.Results:When hydrolyzed by single protease,dedicated protease for silkworm pupa protein showed the highest hydrolysis degree out of 8 proteases used in the experiment,the best hydrolysis conditions were:the substrate concentration was 7％,53℃,pH7.2,the additive amount of protease was 3％,and the hydrolysis degree turned out to be 21.44％.
silkworm pupa proteinenzyme hydrolysisoptimization of conditions
国家科技期刊平台
NSTL
万方数据
维普
