Isolation of Vibrio parahaemolyticus phage and its application in ready-to-eat shrimp
Aimed at using bacteriophage as natural anti-bacteria,this study isolated a lytic Vibrio parahaemolyticus phage through sewage from aquatic market by double-layer method and it was designated as SHOU24,and pH and thermal stability were tested.Phage SHOU24 was specific lytic to Vibrio parahaemolyticus with tdh gene.The phage belonged to Siphoviridae by transmission electron microscope (TEM).The most suitable pH for phage SHOU24 was 7~8 while it could live at pH between 3~11.The survival rate was high under 50℃ condition.In antibacteria on ready-to-eat shrimp experiment,the bacteria amount dropped by 1logCFU/g at 25℃.This study provided theory foundation for using phage SHOU24 as bacterial biocontrol agent.