Geosmin(GSM),produced by microorganism in Daqu,is the important cause of earthy-mouldy offflavor in Chinese liquor.A simple and fast method based on headspace solid-phase microextraction (HS-SPME)coupled with gas chromatography-mass spectrometry(GC-MS)was established for the determination of GSM in Daqu.The analytes were extracted by atmospheric distillation with ethanol solution,and then extracted by HS-SPME using 75μm CAR/PDMS fiber.After concentrating,the sample was analyzed by GC-MS and quantified by the external standard method.The linear ranges were from 100ng/kg to 8000ng/kg for GSM,the correlation coefficient(r) of the calibration curves was 0.999.The recoveries and the relative standard deviations(RSDs) of GSM in Daqu were investigated.The recoveries ranged from 89.19％ to 98.49％ with the RSDs between 2.15％ and 3.06％ (n =5).The limit of detection (LOD) was 42.6ng/kg,and the limit of quantification(LOQ) was 140.6ng/kg.The method is suitable to the determination of GSM in Daqu.
gas chromatography-mass spectrometry (GC-MS)headspace solid-phase microextraction (HS-SPME)geosmin(GSM)Daqu
国家科技期刊平台
NSTL
万方数据
维普
