首页|超声辅助提取对蓝莓花青素提取率、抗氧化活性及化学组成的影响

超声辅助提取对蓝莓花青素提取率、抗氧化活性及化学组成的影响

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采用Box-Behnken设计实验分析超声辅助提取工艺参数对蓝莓花青素的提取率和抗氧化活性的影响,并通过HPLC-ESI-MS法分析超声辅助溶剂浸提对蓝莓花青素化学组成的影响.结果表明:提取率与抗氧化活性的最显著影响因子、最佳工艺参数及回归模型并不相同;提取率的最显著影响因子为超声温度和超声功率强度,最适条件为超声温度39℃、超声功率强度490W、超声pH3.5、超声时间45min;而DPPH·清除率的最显著影响因子为超声时间,最适条件为超声温度45℃、超声功率强度450W、超声pH3.1、超声时间37min;与溶剂浸提法相比,超声辅助溶剂浸提法缩短了浸提时间,但没有改变其化学组成,两种方法都获得1 1种花青素糖苷.
Effect of ultrasonic-assisted extraction on the extraction rate, antioxidant activity and chemical composition of blueberry anthocyanins
In order to obtain the characteristics of the extraction rate,antioxidant activity and chemical composition of blueberry anthocyanins in the ultrasonic assisted extraction,Box-Behnken design,and antioxidant activity test in vitro and HPLC-ESI-MS analysis were employed.The results showed that there were differences in the most significant affecting factors and optimal conditions between the extraction rate and the DPPH scavenging rate of blueberry anthocyanins.The ultrasonic temperature and the ultrasonic power were the most significant factors affecting the extraction rate of blueberry anthocyanins,while the ultrasonic time was the most significant factors affecting the DPPH scavenging rate of blueberry anthocyanins.The optimal conditions for the extraction rate of blueberry anthocyanins were ultrasonic temperature 39℃,ultrasonic power 490W,ultrasonic pH3.5 and ultrasonic time 45min,while for the DPPH scavenging rate of blueberry anthocyanins were ultrasonic temperature 45℃,ultrasonic power 450W,ultrasonic pH3.1 and ultrasonic time 37min.The results of HPLC-ESI-MS analysis showed that 11 anthocyanins from blueberry belonged to anthocyanin glycosides and had the similar chemical composition obtained by both the classic extraction and ultrasound-assisted extraction.

blueberryanthocyaninssolvent macerationultrasonic-assisted extraction

罗水忠、潘利华

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合肥工业大学生物与食品工程学院,安徽合肥230009

安徽省农产品精深加工重点实验室,安徽合肥230009

蓝莓 花青素 溶剂浸提 超声辅助浸提

安徽省2012年度科技攻关项目安徽省2013年度科技攻关计划项目

12010320751301032165

2015

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2015.36(8)
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