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不同干燥方法对淫羊藿多糖化学性质和抗氧化活性的影响

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为研究不同干燥方法对淫羊藿多糖化学性质及抗氧化活性的影响,以粗毛淫羊藿为材料制备多糖.测定热风干燥多糖(EAP-H)、真空干燥多糖(EAP-V)和真空冷冻干燥多糖(EAP-F)的中性糖、糖醛酸和蛋白质含量,单糖组成及DPPH自由基、羟基自由基和超氧阴离子的清除能力.结果表明:淫羊藿多糖为含有少量蛋白质的酸性多糖,不同干燥方法将会影响其单糖组成和糖醛酸含量;EAP-F糖醛酸含量最高并具有最强的DPPH自由基、羟基自由基和超氧阴离子清除能力.因此,真空冷冻干燥是制备淫羊藿多糖的最佳干燥方法.
Effects of different drying methods on chemical properties and antioxidant activities of polysaccharides extracted from Epimedium
The study aimed to explore the effect of drying methods on the chemical properties and antioxidant activities of polysaccharides extracted from Epimedium, three polysaccharides (EAP-H, EAP-V, EAP-F) were obtained from Epimedium acuminatum Franch.via methods such as air drying,vacuum drying and vacuum freeze drying,respectively.Their neutral sugar, uronic acid and protein content were studied, and antioxidant activities were investigated on the basis of assay for DPPH radical, hydroxyl radical, superoxide anion free radical scavenging ability.Results showed that Epimedium polysaccharides were acidic polysaccharides containing a small amount of protein, different drying methods could affect their monosaccharide composition and uronic acid content;compared with air drying and vacuum drying methods, vacuum freeze drying method resulted to Epimedium polysaccharides with higher uronic acid content,stronger antioxidant abilities of DPPH radical, hydroxyl radical and superoxide anion free radical scavenging ability.As a result,vacuum freeze drying method was the best choice for the preparation of the Epimedium polysaccharides.

Epimedium polysaccharideschemical propertiesantioxidant activitiesdrying methods

徐洲、刘静、冯士令、申世安、刘露、向春蓉、丁春邦

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四川农业大学生命科学学院,四川雅安625014

淫羊藿多糖 化学性质 抗氧化活性 干燥方法

四川省科技厅科技支撑计划项目

2014NZ0103

2015

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2015.36(19)
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