Direct competitive ELISA with HRP labeled antigen for determination of furazolidone metabolite residues
In this study,a rapid and high sensitive direct competitive enzyme-linked immunoassay(dc-ELISA) method based on the antigen labeled by Horseradish Peroxidase(HRP) was established,which could be applied to the detection of 3-amino-2-oxazolidinone(AOZ)that is a metabolite of furazolidone in real sample.In the direct competitive assay,monoclonal antibody was bound on the surface of a microtiter plate,then the standards(or the sample) competed with CPAOZ antigen for the antibody binding sites.Calibration curve was prepared for 3-{ [(4-carboxyphenyl) methylene] amino }-2-oxazolidinone (CPAOZ),the IC50 of dcELISA was 0.08 μg/L,the limit of detection was 0.015 μg/L,detection range was 0.025～0.5 μg/L.The recoveries of all kinds of samples were range from 82.0％ to 121.0％.There was almost no cross reaction with other drugs of similar construction.The conclusion suggested that the AOZ-ELISA was a great method with high sensitivity for detecting AOZin samples.
国家科技期刊平台
NSTL
万方数据
维普
