To study the extraction and physicochemical properties of soluble protein from Moringa leaves.Application of ultrasonic and microwave assisted extraction of protein was studied using dry Moringa leaves as a raw material by single factor experiment.The best extraction conditions were determined by Box-Behnken center united experimental design as follows:the ratio for material and liquid was 1∶ 160(g/mL),microwave power of 40 W,extracting time of 127 seconds,pH value of 1 1,under which the yield of protein in the extracts reached 40.11 mg/g.Amino acid analysis showed that content of essential amino acids of Moringa leaf protein was 280.7 mg/g.The content of sulfur amino acid was high.The first limiting amino acid (LAA)was threonine.Differential scanning calorimetry(DSC) analysis showed that the soluble protein thermal denaturation temperature of Moringa leaf was 113.7 ℃.
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