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产酱香功能菌的筛选及其风味物质研究

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本实验从四川某酒厂酱香型大曲中分离得到5株形态各异的菌株,分别进行菌落形态观察、革兰氏染色和感官评价筛选出产酱香菌,然后用HS-SPME结合GC-MS对酱香成分进行分析.结果表明,BL-1、BL-2和BL-5三株菌具有产酱香特性,生理生化分析初步判定三株菌都为枯草芽孢杆菌,HS-SPME和GC-MS检测结果显示,三株菌的酱香成分中吡嗪类、酮类种类和含量都较多,BL-1吡嗪类和酮类的相对含量为75.286%,BL-2的吡嗪类和酮类的相对含量为93.803%,BL-5吡嗪类和酮类的相对含量为86.92%,吡嗪和酮类对酱香风味的贡献较大.
Screening of sauce flavor producing bacteria and flavor compounds research
Five different forms of strain were isolated from a winery in Sichuan sauce flavor Daqu,colony morphology,Gram stain and sensory evaluation of which were conducted and sauce producing bacteria was screened,and the sauce flavor compounds were analyzed by HS-SPME combined with GC-MS.The results showed that,BL-1,BL-2 and BL-5 could produce sauce flavor,which were Bacillus subtilis analyzed by the physiological and biochemical mechods.The results detected by HS-SPME and GC-MS showed that the type and content of pyrazine and ketones were more than others among the sauce ingredients of the three strain.The relative content of pyrazines and ketones in BL-1 was 75.286%,and the relative content of pyrazines and ketones in BL-2 was 93.803%,and the relative content of pyrazines and ketones in BL-5 was 86.92%,which showed that pyrazine and ketones were greater contribution on producing sauce flavor than others.

Bacillus subtilissauce flavorGC-MS

赵兴秀、何义国、赵长青、方春玉、张静、邹伟

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四川理工学院生物工程学院,四川自贡643000

枯草芽孢杆菌 酱香 GC-MS

酿酒生物技术及应用四川省重点实验室项目泸州老窖科研奖学金项目四川省教育厅项目绿色催化四川省高校重点实验室项目四川理工学院科研项目四川理工学院科研项目

NJ2012-1613ljzk0514ZA0206LYJ13042012PY082015PY02

2016

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2016.37(6)
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