以总花色苷含量和DPPH·清除率为评价指标,在考察总花色苷含量测定方法的基础上,对黑枸杞花色苷的提取工艺进行优化.经单因素实验和L9(34)正交实验,选择提取方式为静置法,提取溶剂为1％甲酸,提取温度为75℃,料液比为1∶30 g/mL,每次提取时间为1h和提取次数为3次.优化工艺的总花色苷含量和DPPH·清除率分别达到(450.55 ±5.91) mg/100 g DW和72.53％±0.60％,相比基础工艺分别提高了17.22％和21.96％.
Optimization of the extraction process of anthocyanins in Lycium ruthenicum Murr.
Taken total anthocyanin content and DPPH radical scavenging ratio as indexes,the extraction process of the anthocyanins in Lycium ruthenicum Murr.' s fruit (LRMF) had been optimized.By univariate experiment and L9 (34) orthogonal test,the selected condition was determined as follows:static extraction (extraction mode),1％ formic acid(extraction solvent),75 ℃ (extraction temperature),1:30 g/mL (solid-liquid ratio),1 h (extraction time) and 3 times(extraction frequency).Verification experiment demonstrated that the total anthocyanins content and DPPH radical scavenging ratio of the optimal process was(450.55 ±5.91)mg/100 g DW and 72.53％ ±0.60％,respectively,increased by 17.22％ and 21.96％ in contrast to basic process.
Lycium ruthenicum Murr.anthocyanintotal anthocyanin contentDPPH radical scavenging ratio
国家科技期刊平台
NSTL
万方数据
维普
