Studies on Daqu microbial community structure based on phospholipid fatty acid (PLFA)analysis
The community diversity of microbial in sauce-flavor Daqu,strong-flavor Daqu and light-flavor Daqu were investigated using phospholipid fatty acids (PLFA) as a culture-independent method.The results showed that according to phospholipid fatty acid(PLFA) technique,a total of 18 fatty acids with chain lengths ranging from Ci4 to C20 were detected,including straight-chain PLFA,branched PLFA,monounsaturated PLFA,and the polyunsaturated PLFA.The amount of fungi dominated in the different Daqu.Specifically,gram-positive bacteria (G +) were the main bacteria,the 18∶2ω6,9 and 18∶1ω9 accounted for more than 90% PLFA.The correlation between the craftwork of Daqu and the microbial community structure was obvious.The community diversity was decreased as the craftwork temperature of Daqu increasing.