首页|基于磷脂脂肪酸分析技术的大曲微生物群落结构多样性研究

基于磷脂脂肪酸分析技术的大曲微生物群落结构多样性研究

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采用磷脂脂肪酸(PLFA)技术,对清香型大曲、浓香型大曲和酱香型大曲微生物群落结构及其多样性进行研究.结果表明,基于磷脂脂肪酸分析技术在不同类型大曲中共检出碳原子总数从14到20的PLFA 18种,主要包括直链饱和脂肪酸、支链饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸.大曲微生物都以真菌(18∶2ω6,9、18∶ 1ω9)含量为主,占脂肪酸总量的90%以上,大曲中细菌微生物主要以G+(a14∶0、i14∶0、i15∶0、a16∶0、i16∶0)为主;不同工艺大曲微生物群落结构存在明显差异,随着制曲温度的升高,大曲微生物多样性指数有下降趋势.
Studies on Daqu microbial community structure based on phospholipid fatty acid (PLFA)analysis
The community diversity of microbial in sauce-flavor Daqu,strong-flavor Daqu and light-flavor Daqu were investigated using phospholipid fatty acids (PLFA) as a culture-independent method.The results showed that according to phospholipid fatty acid(PLFA) technique,a total of 18 fatty acids with chain lengths ranging from Ci4 to C20 were detected,including straight-chain PLFA,branched PLFA,monounsaturated PLFA,and the polyunsaturated PLFA.The amount of fungi dominated in the different Daqu.Specifically,gram-positive bacteria (G +) were the main bacteria,the 18∶2ω6,9 and 18∶1ω9 accounted for more than 90% PLFA.The correlation between the craftwork of Daqu and the microbial community structure was obvious.The community diversity was decreased as the craftwork temperature of Daqu increasing.

Daquphospholipid fatty acidmicrobial community

赵金松、郑佳、沈才洪、周荣清

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四川理工学院,四川自贡643000

四川大学轻纺与食品学院,四川成都610065

国家固态酿造工程技术研究中心,四川泸州646000

大曲 磷脂脂肪酸 微生物群落

国家自然科学基金资助四川理工学院人才引进项目

311717422014RC27

2017

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2017.38(1)
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