Technology optimization for enzymatic preparation of κ-carrageenan oligosaccharides
In this study,κ-carrageenan oligosaccharides were prepared by enzymatic hyrolysis method to improve the technology.Hydrolysis conditions were optimized with the characteristic signs of reducing sugars content,and the optimal conditions for κ-carrageenan oligosaccharides preparation were obtained as substrate concentration 12 g/L,pH7.0,reaction temperature 40 ℃ and shaking speed 100 r/min,respectively.Compared with the reducing sugars yield of the initial conditions,the yield of the optimal conditions increased from 0.91 mg/mL to 1.61 mg/mL,which was increased by 77％.The Km and Vmax values of the enzyme hydrolysis reaction was 171.96 mg/mL and 0.925 mg· mL-1 · min-1,respectively.Then,the result of optimal process validation test could consistent with that of 20 L enlarge experiment.At last,the enzymatic hydrolysis products were analyzed by thin-layer chromatography and mass spectrometry,the result showed that the main products included tetrasaccharide and hexasaccharides in 24 h reaction.
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