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酶转化在淫羊藿苷提取中的应用

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目前生产淫羊藿苷的过程中,其副产物朝藿定A、B、C等黄酮未被利用好.筛选出了将淫羊藿苷废渣中朝藿定A、B、C等黄酮转化成淫羊藿苷的A.sp.y39菌酶,将其应用于以淫羊藿提取物为原料制备淫羊藿苷的工艺.将150 g西安岳达淫羊藿提取物,溶解于60%乙醇、经D-280脱色柱脱色、结晶得到30.5 g淫羊藿苷;其废渣朝藿定A、B、C混合黄酮,经A.sp.y39菌酶转化,又得到16.6 g淫羊藿苷.共得到纯度90%以上的淫羊藿苷47.1 g;淫羊藿苷的提取率由传统的20.3%提高到31.4%;相比于传统方法,淫羊藿苷的提取率提高了50%以上.本文为节约淫羊藿资源提供了依据.
Bioconversion utilization of waste-residue from icarrin extraction
In current domestic situation,the by-products containing with flavonoids such as epimedin A,B,C was not being used during the icarrin production from epimedium extract.In this paper,the enzyme from A.sp.y 39 strain was filtered for transformating epimedin A,B,C in waste offscum of epimedium extract.The 150 g of the market epimedium extract was dissolved in 60% ethyl alcohol for decoloring by D-280 macroporous resin,and then 30.5 g of icariin was obtained from above discolored epimedium extract by crystallization method.Then,16.6 g of icariin was obtained from the waste offscum containing epimedin A,B and C by the enzyme from A.sp.y39 strain.Finally,47.1 g of icariin(the purity was over 90%) was obtained from 150 g of epimedium extract by above enzyme transformation methods,the yield of icariin was rised from 20.3% to 31.4%.Compared with the traditional method,the extraction rate of icariin was increased by more than 50%,which provided the basis for saving epimedium resources.

eptimedium extractepimedium flavonoidsicariinepimendin A, B, C

富盈昕、刘春莹、郭美娟、赵耀、金凤燮、鱼红闪

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大连工业大学生物工程学院,辽宁大连116034

淫羊藿提取物 淫羊藿黄酮苷酶 淫羊藿苷 朝藿定A、B、C

“重大新药创新”科技重大专项国家高端外国专家项目

2012ZX09503001-003GDT20152100019

2017

食品工业科技
北京一轻研究院

食品工业科技

CSTPCDCSCD北大核心
影响因子:0.842
ISSN:1002-0306
年,卷(期):2017.38(8)
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