Bioconversion utilization of waste-residue from icarrin extraction
In current domestic situation,the by-products containing with flavonoids such as epimedin A,B,C was not being used during the icarrin production from epimedium extract.In this paper,the enzyme from A.sp.y 39 strain was filtered for transformating epimedin A,B,C in waste offscum of epimedium extract.The 150 g of the market epimedium extract was dissolved in 60% ethyl alcohol for decoloring by D-280 macroporous resin,and then 30.5 g of icariin was obtained from above discolored epimedium extract by crystallization method.Then,16.6 g of icariin was obtained from the waste offscum containing epimedin A,B and C by the enzyme from A.sp.y39 strain.Finally,47.1 g of icariin(the purity was over 90%) was obtained from 150 g of epimedium extract by above enzyme transformation methods,the yield of icariin was rised from 20.3% to 31.4%.Compared with the traditional method,the extraction rate of icariin was increased by more than 50%,which provided the basis for saving epimedium resources.
eptimedium extractepimedium flavonoidsicariinepimendin A, B, C