贻贝仿生涂层修饰聚合物微球固定化脂肪酶的制备及应用
Synthesis and Application of Lipase Immobilized on Mussel-inspired Polymer Microspheres
王淑婧 1曾祥冰 1孙西同 2陈晓艺 1李苗 1王添誉 1李岩 1李佥 1李宪臻1
作者信息
- 1. 大连工业大学生物工程学院,辽宁大连 116034
- 2. 大连工业大学轻工与化学工程学院,辽宁大连 116034
- 折叠
摘要
为构建新型固定化酶催化体系,以聚甲基丙烯酸缩水甘油酯为载体,利用贻贝仿生技术——多巴胺/聚乙烯亚胺共沉积进行修饰,采用扫描电镜(SEM)、能谱(EDS)、Zeta电位及红外光谱(FT-IR)表征所得材料,并研究其固定化近平滑假丝酵母CICC 33470所产脂肪酶的表征及酶学性质.最佳酶固定化条件为:固定化温度为30℃,固定化pH为 7.0,固定化时间为 5h,初始酶活为 337.76 U/mL,载体添加量为 0.2 g.固定化酶最佳反应温度为 50℃,最佳反应pH为 8.0,最佳反应时间为 10 min,最优条件下固定化酶酶活为 484.42±5.97 U/g-载体.固定化酶的稳定性明显提高,重复使用 8次后,固定化酶仍有 39.22%的初始酶活.进一步将固定化酶用于催化乙酰丙酸与十二醇的酯化反应,转化率可达 75.94%,充分证明聚甲基丙烯酸缩水甘油酯经修饰后是固定化脂肪酶的优良载体,为未来扩大脂肪酶的应用范围提供了基础数据.
Abstract
In order to construct a new type of immobilized enzyme catalytic system,poly(glycidyl methacrylate)was used as a support,and was modified with dopamine/polyethyleneimine by the mussel-inspired co-deposition method.The resulted material was characterized by scanning electron microscope(SEM),energy spectroscopy(EDS),Zeta potential and Fourier transform infrared spectrometer(FT-IR),respectively.Then,the resulted material was applied for the immobilization of lipase,which was fermented via Candida parapsilosis CICC 33470.The characterization and enzymatic properties of immobilized enzyme were also investigated.The optimal immobilized conditions were the immobilization temperature of 30℃,the immobilization pH of 7.0,the immobilization time of 5 h and the immobilization initial enzyme acticity of 337.76 U/mL,together with the carrier addition of 0.2 g.It was indicated that the maximum enzyme activity reached 484.42±5.97 U/g-support,as the optimal reaction temperature,reaction pH and reaction time were 50℃,8.0 and 10 min,respectively.In addition,the immobilized lipase exhibited excellent stability,and it kept 39.22%of original enzyme activity after eight reuse cycles.Furthermore,when the immobilized lipase was used to catalyze the esterification reaction of levulinic acid and lauryl alcohol,the conversion rate could reach 75.94%.It was illuminated that the modified poly(glycidyl methacrylate)could serve as a good support for lipase immobilization,and the results provided basic data for expanding the application areas of lipase in the future.
关键词
甲基丙烯酸缩水甘油酯/固定化脂肪酶/多巴胺/聚乙烯亚胺共沉积/近平滑假丝酵母/酯化反应Key words
glycidyl methacrylate/lipase immobilization/dopamine/polyethyleneimine co-deposition/Candida parapsi-losis/esterification引用本文复制引用
基金项目
国家自然科学基金青年基金(31601411)
国家自然科学基金青年基金(21804129)
国家自然科学基金青年基金(22108024)
辽宁省教育厅科学研究项目(JYTMS20230423)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据