落叶松中花旗松素的提取、抑菌作用及其与白屈菜红碱联用协同抑菌作用机理
Extraction,Bacteriostatic Effect and Synergistic Mechanism of Bacteriostatic Effect of Taxifolin in Larch in Combination with Leucocyanidin
荆立成 1杨跃 1杨阔 1李玫萱 1赵敏 1崔岱宗1
作者信息
- 1. 东北林业大学生命科学学院,黑龙江哈尔滨 150036
- 折叠
摘要
本研究以落叶松为原料,采用超声辅助乙醇热浸提法提取花旗松素.以大肠杆菌和金黄色葡萄球菌分别作为典型供试菌,通过观察细菌形态结构,测定细菌生长量、细胞膜泄漏、抗氧化酶系活性等变化情况,分析花旗松素抑菌效果.最终获得纯度达 90%、提取率 0.35%的花旗松素,且花旗松素对两种菌株的最小抑菌浓度均为1.2 mg/mL,抑菌率分别为81.12%、83.95%.通过电镜扫描后发现菌体表面被破坏伴有内容物泄漏.在Escherichia coli中,培养至 24h的 1/2 MIC、1 MIC和 2 MIC实验组OD260 测试值是同期对照组的 1.18、1.52、1.88倍,检测到胞外β-半乳糖苷酶的相对活性分别为 79.15%和 70.1%.Bliss独立模型计算生长速率为 0.0142,表明花旗松素和白屈菜红碱药物联用后对金黄色葡萄球菌表现为协同抑制作用.药物联用条件下,低浓度对细菌细胞膜破坏明显增强,抑菌效果更加显著,培养上清液中β-半乳糖苷酶活达 87.01%,较 2 MIC单独作用S.aureus酶活性高16.91%,24 h CAT、SOD和POD酶活较单独使用 1 MIC浓度花旗松素对S.aureus作用要更高,分别是其 1.28、1.25、1.11倍.研究将为花旗松素作为食品保鲜、防腐剂等产品的开发应用奠定理论基础.
Abstract
Using larch as raw material,taxifolin was extracted by ultrasonic-assisted ethanol hot leaching.Escherichia coli and Staphylococcus aureus were used as typical test bacteria respectively,and the bacterial inhibitory effects of taxifolin was analyzed by observing the morphological structure of bacteria and measuring the changes in bacterial growth,cell membrane leakage and antioxidant enzyme system activities.The final product was obtained with 90%purity and 0.35%extraction rate of taxifolin.The minimum inhibitory concentration of taxifolin was 1.2 mg/mL for both strains,and the inhibition rate was 81.12%and 83.95%,respectively.After the electron microscopy analysis,the surface of the bacteria cells was disrupted and the contents inside the cells were leaked.In E.coli,the OD260 test values of 1/2 MIC,1 MIC and 2 MIC were 1.18,1.52 and 1.88 times higher than those of the control group after 24 h incubation.The relative activities of extracellular β-galactosidase were 79.15%and 70.1%,respectively,and the growth rate calculated by the the Bliss independent model was 0.0142.S.aureus showed a synergistic inhibition effect.Under the conditions of drug combination,the bacterial cell membrane disruption extent was significantly enhanced by the low concentration and the inhibition effect was more significant.The β-galactosidase activity in the culture supernatant was 87.01%higher than that of 2 MIC group alone for S.aureus by 16.91%,and the 24 h CAT,SOD and POD enzyme activities were higher than that of 1 MIC group alone for S.aureus,which were 1.28,1.25 and 1.11 times higher,respectively.The study could lay the theoretical foundation for the development and application of taxifolin as a food preservation and preservative.
关键词
花旗松素/抑菌活性/药物联用/抑菌机制Key words
taxifolin/antibacterial activity/drug combination/mechanism of inhibition引用本文复制引用
基金项目
中央高校基本科研业务费专项资金项目(2572020DR08)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据