蜜环菌Am-07-22发酵对玉米赤霉烯酮降解效果影响及机理初探
Effect and Mechanism of Armillaria mellea07-22 Fermentation on the Degradation of Zearalenone
王泽贤 1赵宇楠 1贾丹丹 1纪晚唐 1许丁 1向杨玲 1蔡丹 1刘景圣1
作者信息
- 1. 吉林农业大学食品科学与工程学院,小麦和玉米深加工国家工程研究中心,吉林长春 130118
- 折叠
摘要
本研究以蜜环菌Am-07-22为试验菌株,采用真菌生物发酵的方式降解玉米赤霉烯酮(ZEN),对蜜环菌降解ZEN的降解效果进行研究,包括菌株对不同浓度ZEN的降解效果以及不同培养时间、培养温度、初始pH和接种量对菌株降解ZEN的影响.然后对降解机理进行初探,分析了菌丝体、发酵上清液、细胞内容物对ZEN的降解作用,并研究了不同发酵时间、pH、金属离子对发酵上清液降解ZEN的影响,以及降解效果与菌株产漆酶活力的相关性分析.结果表明:蜜环菌Am-07-22 对ZEN的降解效果良好,当ZEN浓度为 5 μg/mL时,最适降解条件为培养时间 8d,培养温度 27℃,初始pH7.0,接种量 10%,此时对ZEN的降解率为 78.72%.菌丝体、发酵上清液和细胞内容物对ZEN的降解率分别为 47.42%、37.05%和 13.08%,蜜环菌Am-07-22分泌的胞外酶是降解ZEN的主要方式,而且菌体细胞对ZEN也有一定的吸附作用.另外,发酵上清液对ZEN的降解率与漆酶酶活的相关性较高为 0.973,且Cu2+对发酵上清液降解ZEN具有最佳的促进作用.
Abstract
This study used Armillaria mellea 07-22 as the experimental strain to degrade zearalenone(ZEN)by fungal biological fermentation.The degradation effects of Armillaria mellea on ZEN were studied,including the degradation effects of different concentrations of ZEN by the strain and the effects of different culture time,culture temperature,initial pH value and inoculation amount on the degradation of ZEN by the strain.Then the degradation mechanism was explored,the degradation effects of mycelium,fermentation supernatant and cell contents on ZEN were analyzed,and the effects of different fermentation time,pH values,and metal ions on degradation of ZEN by fermentation supernatant were studied,and the correlation between degradation effect and laccase production activity of the strain was illustrated.The results showed that Armillaria mellea07-22 had a good degradation effect on ZEN.When the ZEN concentration was 5 μg/mL,the optimal degradation conditions were culture time of 8 days,culture temperature of 27℃,initial pH of 7.0,and inoculation amount of 10%.At this time,the degradation rate of ZEN was 78.72%.The degradation rates of ZEN by mycelium,fermentation supernatant and cell contents were 47.42%,37.05%and 13.08%respectively.The extracellular enzymes secreted by Am-07-22 were the main way to degrade ZEN,and the mycelium cells also had a certain adsorption effect on ZEN.In addition,the correlation between the degradation rate of ZEN by fermentation supernatant and laccase activity was 0.973,and Cu2+ had the best promoting effect on the degradation of ZEN by fermentation supernatant.
关键词
玉米赤霉烯酮/蜜环菌/真菌毒素/微生物降解/降解机理Key words
zearalenone/Armillaria mellea/mycotoxin/microbial degradation/degradation mechanism引用本文复制引用
基金项目
吉林省科技发展计划项目(20210203124SF)
吉林省中青年科技创新创业卓越人才(团队)项目(创新类)(20230508014RC)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据