乳双歧杆菌Y6活菌型发酵乳对消化功能障碍人群肠道健康的影响
Effects of Fermented Milk with Live Bifidobacterium lactis Y6 on Intestinal Health in People with Digestive Dysfunction
黎旭 1黄志钰 1黄娟 2陶纯长 1金庭飞1
作者信息
- 1. 广东益可维生物技术有限公司,广东广州 510630
- 2. 广东燕塘乳业股份有限公司,广东广州 511356
- 折叠
摘要
为了探究饮用乳双歧杆菌Y6活菌型发酵乳对消化功能障碍人群肠道健康的影响,本文进行了一项膳食干预试验.选择具有消化功能问题的受试者,每天饮用 200 mL Y6活菌型发酵乳,持续 4周.分别在干预前后进行临床评分,同时利用Illumina PE300测序平台,对受试者粪便微生物的 16S rDNA PCR产物片段进行高通量测序,使用气相色谱测定了粪便中短链脂肪酸(SCFAs)的含量.结果表明,饮用Y6活菌型发酵乳对消化功能障碍导致的临床症状有极显著的改善作用(P<0.01);高通量测序结果显示,Y6活菌型发酵乳的干预对人体肠道微生物群的组成影响显著,但对物种多样性和丰富度没有明显改变,其中有益菌Akkermansia、Collinsella和Erysipelotrichacee_UCG_003属的相对丰度增加,有害菌Lachnoclostridium属的相对丰富度减少;肠道内SCFAs(乙酸、丙酸和丁酸)含量显著增加(P<0.05).乳双歧杆菌Y6 活菌型发酵乳的干预可以显著改善消化功能障碍人群的肠道健康,为Y6菌株的应用提供理论依据.
Abstract
To investigate the effects of administering fermented milk with live Bifidobacterium lactis Y6 on intestinal health in people with digestive dysfunction,a dietary intervention experiment was conducted.Volunteers with digestive problems were selected to consume 200 mL Y6 fermented milk daily for 4 weeks.Clinical scores were performed before and after the intervention,the Illumina PE300 sequencing platform was used to perform high-throughput sequencing of 16S rDNA PCR product fragments of fecal microorganisms in volunteers,the content of short-chain fatty acids(SCFAs)in feces was determined using gas chromatography.The results showed that drinking Y6 fermented milk had a significant improvement on clinical symptoms caused by digestive dysfunction(P<0.01).High-throughput sequencing results showed that drinking Y6 fermented milk had significant effect on the composition of gut microbiota rather than the diversity and richness.The relative abundance of beneficial bacteria Akkermansia,Collinse and Erysipelotrichaceae_UCG_003 genera increased while the harmful bacteria Lachnoclostridium genera reduced.The contents of intestinal SCFAs(acetic acid,propionic acid and butyric acid)increased significantly(P<0.05).The intervention of fermented milk with live Bifidobacterium lactis Y6 can significantly improve the intestinal health of people with digestive dysfunction,providing a theoretical basis for the application of Y6 strains.
关键词
乳双歧杆菌Y6/活菌型发酵乳/消化功能障碍/短链脂肪酸(SCFAs)/肠道健康Key words
Bifidobacterium lactis Y6/fermented milk with live cultures/digestive dysfunction/short-chain fatty acid(SCFAs)/intestinal health引用本文复制引用
基金项目
新疆维吾尔自治区区域协同创新专项(上海合作组织科技伙伴计划及国际科技合作计划)项目(2022E01028)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据