摘要
为增强花色苷的稳定性,拓宽其在食品保健以及医药领域的应用,以羧甲基壳聚糖(CMCS)、L-组氨酸(L-His)和硬脂酸(SA)为载体材料,蓝靛果果渣花色苷为芯材,采用响应面法优化酸响应性蓝靛果果渣花色苷胶束的制备工艺,通过在光照、温度、pH等不同条件下蓝靛果果渣花色苷的稳定性以及其对肝癌细胞杀伤性进行评价.结果表明:经响应面优化的最佳制备工艺为水浴时间 10 min,超声时间 15 min,超声温度 60℃,包封率为 85.3%±0.31%.储存稳定性分析表明在各种温度、pH、光照、氧化剂、还原剂的条件下,载花色苷胶束中花色苷的保存率分别是花色苷的 1.33、1.26、1.16、1.11、2.41倍;在体外肝癌细胞杀伤实验中,在 24h后,载花色苷胶束对肝癌细胞抑制率是花色苷的 1.34倍.本研究合成了一种新型的两亲性羧甲基壳聚糖衍生物,可以显著提高花色苷稳定性,适宜作为花色苷以及其他食品活性成分的潜在纳米胶束载体.
Abstract
In order to enhance the stability of anthocyanins and broaden its application in the fields of food health care and medicine,carboxymethyl chitosan(CMCS),L-histidine(L-His)and stearic acid(SA)were used as carrier materials,and Lonicera edulis pomace anthocyanins were used as core materials.The preparation process of acid-responsive Lonicera edulis pomace anthocyanins micelles was optimized by response surface method.The stability of Lonicera edulis pomace anthocyanins under different conditions such as light,temperature and pH and their cytotoxicity to liver cancer cells were evaluated.The results showed that the optimal preparation process was as follows:Water bath time 10 min,ultrasonic time 15 min,ultrasonic temperature 60 °C,and the encapsulation efficiency was 85.3%±0.31%.The storage stability analysis showed that under various conditions of temperature,pH,light,oxidant and reducing agent,the preservation rate of anth-ocyanins in the micelles were 1.33,1.26,1.16,1.11 and 2.41 times that of anthocyanins,respectively.In the in vitro liver cancer cell killing experiment,after 24 h,the inhibition rate of anthocyanin-loaded micelles on liver cancer cells was 1.34 times that of anthocyanin.In this study,a novel amphiphilic carboxymethyl chitosan derivative was synthesized,which could significantly improve the stability of anthocyanins and was suitable as a potential nanomicelle carrier for anthocya-nins and other food active ingredients.
基金项目
黑龙江省高校基本科研业务费黑龙江大学专项资金项目(2021KYYWF0046)
黑龙江省博士后面上项目(LBHZ22040)
黑龙江省高校基本科研业务费黑龙江大学专项资金项目(2022KYYWF1078)
国家科技期刊平台
NSTL
万方数据