褪黑素对青椒低温贮藏品质及能量代谢影响
Effects of Melatonin on Storage Quality and Energy Metabolism of Green Pepper(Capsicum frutescens L.)During Low Temperature Storage
黄添舆 1罗冬兰 1王小岗 1李江阔 2王秀芬 1曹森1
作者信息
- 1. 贵阳学院食品科学与工程学院,贵州省果品加工工程技术研究中,贵州贵阳 550005
- 2. 国家农产品保鲜工程技术研究中心(天津),农业农村部农产品贮藏保鲜重点实验室,天津市农产品采后生理与贮藏保鲜重点实验室,天津 300384
- 折叠
摘要
为研究褪黑激素对采后青椒果实贮藏品质和能量代谢的影响,本研究以青椒为材料,研究了 100、200和300 μmol/L浓度褪黑素对 9℃下贮藏的青椒果实品质及能量代谢的影响.结果表明,褪黑素处理能有效地降低青椒果实腐烂率,减缓青椒果实硬度的下降,其中,200 μmol/L褪黑素的处理效果最好;此外,褪黑素能推迟青椒果实辣椒碱含量、叶绿素含量的下降,维持较低的丙二醛(MDA)含量,抑制青椒三磷酸腺苷(ATP)、二磷酸腺苷(ADP)和腺嘌呤核苷酸(AXP)的降解,促进一磷酸腺苷(AMP)含量的积累,维持了较高的能荷水平.褪黑素也能维持青椒中的较高的琥珀酸脱氢酶(SDH)活性、细胞色素C氧化酶(CCO)活性、氢离子ATP酶(H+-ATPase)酶活性和钙离子ATP酶(Ca2+-ATPase)酶活性.相关性分析表明,青椒的ATP、ADP、AXP、能荷、H+-ATPase、Ca2+-ATPase活性与腐烂率呈显著负相关(P<0.05),与辣椒碱和叶绿素含量呈显著正相关(P<0.05),并且青椒的ATP、ADP、AMP、AXP、能荷和H+-ATPase、Ca2+-ATPase活性互相之间均呈现显著正相关(P<0.05),表明褪黑素处理能够说明采后青椒贮藏期品质与能量水平紧密相关.研究结果可为褪黑素在控制采后青椒贮藏品质方面的应用提供技术参考.
Abstract
In order to study the effect of melatonin on the storage quality and energy metabolism of postharvest green pep-per(Capsicum frutescens L.).The green pepper was used as the experiment material in this experiment with different concentrations(100,200 and 300 μmol/L)at low temperature(9℃).To investigate the effects of melatonin treatment on the storage quality and energy metabolism of green pepper fruits.The results showed that melatonin treatment could effe-ctively reduce decay rate and slow down the decline of fruit hardness of green pepper.In particular,the treatment effect of 200 μmol/L melatonin was the best.In addition,melatonin could delay the decline of capsaicin and chlorophyll content,mai-ntained a lower MDA content,inhibited the degradation of ATP,ADP and AXP,promoted the accumulation of AMP con-tent,and maintained a higher energy charge level in green pepper.Melatonin could also maintain high SDH,CCO,H+-ATPase and Ca2+-ATPase activity in green pepper.Correlation analysis showed that the activities of ATP,ADP,AXP,energy charge,H+-ATPase and Ca2+-ATPase in green pepper were significantly negatively correlated with the decay rate(P<0.05),and were significantly positively correlated with the contents of capsaicin and chlorophyll(P<0.05).Moreover,the activities of ATP,ADP,AMP,AXP,energy charge,H+-ATPase and Ca2+-ATPase in green pepper were significantly positively correlated with each other(P<0.05),indicating that melatonin treatment could explain that the quality of post-harvest green pepper was closely related to energy level during storage.The results of this study can provide technical refe-rence for the application of melatonin in controlling the storage quality of postharvest green pepper.
关键词
青椒/褪黑素/贮藏品质/能量代谢/相关性分析Key words
Capsicum frutescens/melatonin/storage quality/energy metabolism/correlation analysis引用本文复制引用
基金项目
贵州省科技计划项目(黔科中引地[2022]4050)
贵阳市科技计划项目(筑科合同[2022]3-17号)
2022年贵阳学院硕士研究生科研基金项目(GYU-YJS[2022]-14)
出版年
2024
国家科技期刊平台
NSTL
万方数据