椰毒假单胞菌酵米面亚种产毒差异蛋白质组学分析
Proteomic Analysis of Difference in Toxin Production of Pseudomonas cocovenenans subsp.farinofermentans
黄秀丽 1沈圣 2朱文娟 1陈佳平 3陈国培 3赵智锋 1黄永德 1陈嘉聪 1温晓裕1
作者信息
- 1. 惠州市食品药品检验所,广东惠州 516003
- 2. 惠州市市场监督管理信息中心,广东惠州 516003
- 3. 深检集团(深圳)医学检验实验室,广东深圳 518131
- 折叠
摘要
为了从蛋白质水平探索椰毒假单胞菌米酵菌酸毒素产生机制,采用数据依赖型采集(data dependent acquisition,DDA)非标记定量蛋白质组学技术对该菌产毒培养前后蛋白质变化进行了分析.结果显示:产毒培养后,产毒株中显著性上调表达蛋白 25个,显著性下调表达蛋白 31个,其中显著性上调表达的蛋白中有与细菌趋化性信号转导相关的ABC转运蛋白、反应调节受体蛋白、甲基受体趋化性蛋白Ⅱ,以及与细菌运动相关的鞭毛蛋白如鞭毛P环蛋白、鞭毛M环蛋白、鞭毛钩蛋白FlgE等,推测趋化性运动可能在椰毒假单胞菌米酵菌酸毒素产生过程中发挥重要作用.椰毒假单胞菌米酵菌酸毒素产生机制研究,可为人们更好地降低食源性疾病的发生提供理论支撑.
Abstract
To study the mechanism of bongkrekic acid production by Pseudomonas cocovenenans subsp.farinofermentans at proteome level,data-dependent acquisition(DDA)unlabeled quantitative proteomics technique was used to analyze the protein changes of P.cocovenenans before and after toxin production.The results showed that 25 proteins were up-regulated and 31 proteins were down-regulated in toxin-producing strains.Among them,ABC transporters,response regulatory receptor protein,methyl-accepting chemotaxis protein Ⅱ were related to bacterial chemotactic signal transduction.And flagellins such as P-cyclin,M-cyclin and FlgE were all related to chemotaxis.It is supposed that chemotactic movement plays an important role in the production of bongkrekic acid.The study on the production mechanism of bongkrekic acid can provide theoretical evidence to decrease the incidence of foodborne diseases.
关键词
DDA非标记定量蛋白质组学/椰毒假单胞菌/米酵菌酸/产毒机制/食物中毒Key words
DDA unlabeled quantitative proteomics/Pseudomonas cocovenenans subsp.farinofermentans/bongkrekic acid/mechanism of toxin production/food poisoning引用本文复制引用
基金项目
惠州市科技计划项目(2021WC010301)
惠州市科技计划项目(2020SC0204013)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据