两株代谢咖啡酸人肠道来源菌株的筛选、鉴定及其代谢过程初探
Screening and Identification of Two Human Intestinal Strains Metabolizing Caffeic Acid and Exploring Their Metabolic Processes
杨雯 1胡海明 2刘洪涛 2魏晓博 1刘慧燕 1方海田1
作者信息
- 1. 宁夏大学食品科学与工程学院,宁夏食品微生物应用技术与安全控制重点实验室,宁夏银川 750021
- 2. 湖北中医药大学基础医学院,湖北武汉 430065
- 折叠
摘要
为了探索咖啡酸的体内代谢过程,本研究从人体肠道粪便中分离筛选出 2株能代谢咖啡酸的菌株,通过细胞形态、16S rDNA序列和系统发育树分析了其菌株特征,并研究了两株菌株代谢咖啡酸过程中的细菌总数和pH变化,最后采用薄层层析法和高效液相色谱法对代谢产物进行确定,阐明咖啡酸代谢过程.结果表明,2株菌分别为腐生葡萄球菌(Staphylococcus xylosus)和奇异变形杆菌(Proteus mirabilis).腐生葡萄球菌代谢咖啡酸含量从 0.539 mg/mL在 6h后降至 0.087 mg/mL,12 h后完全被代谢.而奇异变形杆菌,在 6h后咖啡酸含量从0.543 mg/mL降至 0.352 mg/mL,24 h后完全被代谢.咖啡酸抑制了肠道菌的增殖,且腐生葡萄球菌代谢咖啡酸能力优于奇异变形杆菌,肠道菌代谢咖啡酸产物为间香豆酸.研究结果为进一步研究咖啡酸和肠道菌的相互作用提供依据.
Abstract
In order to explore the metabolic process of caffeic acid in vivo,two strains that could metabolize caffeic acid were isolated and screened from human intestinal feces,their strain characteristics were analyzed by cell morphology,16S rDNA sequence and phylogenetic tree,and the total number of bacteria and pH changes in the process of caffeic acid metabolism in the two strains were studied,and finally the metabolites were determined by thin layer chromatography and high performance liquid chromatography to elucidate the caffeic acid metabolism process.The results showed that the two strains were Staphylococcus xylosus strain and Proteus mirabilis strain.The content of caffeic acid metabolized by Staphylococcus xylosus strain decreased from 0.539 mg/mL to 0.087 mg/mL after 6 h and was completely metabolized after 12 h.However,the content of caffeic acid metabolized by Proteus mirabilis strain decreased from 0.543 mg/mL to 0.352 mg/mL after 6 h,and was completely metabolized after 24 h.Caffeic acid inhibited the proliferation of intestinal bacteria,and the ability of Staphylococcus xylosus strain to metabolize caffeic acid was better than that of Proteus mirabilis strain,and the product of caffeic acid metabolized by intestinal bacteria was m-coumaric acid.The results of the study provided a basis for further research on the interaction between caffeic acid and intestinal bacteria.
关键词
咖啡酸/腐生葡萄球菌/奇异变形杆菌/筛选/代谢产物Key words
caffeic acid/Staphylococcus xylosus strain/Proteus mirabilis strain/screening/metabolites引用本文复制引用
基金项目
宁夏重点研发计划项目(2018BBF02008)
宁夏食品微生物应用技术与安全控制重点实验室平台建设项目(2021JCTJ0030)
自治区青年拔尖人才工程(2019)()
宁夏大学贺兰山学者项目(2020)()
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据