昆布多糖的复合酶法提取工艺优化及其对α-葡萄糖苷酶的抑制活性
Optimization of Multi-Enzyme-Assisted Laminarin Extraction and Evaluation of Laminarin Inhibitory Effect on α-Glucosidase Activity
张涵 1殷澳 2张会佳 1侯相竹 2高阳 1徐多多2
作者信息
- 1. 长春中医药大学人参科学研究院,吉林长春 130117
- 2. 长春中医药大学药学院,吉林长春 130117
- 折叠
摘要
优化复合酶提取昆布多糖的工艺参数,并考察其抑制α-葡萄糖苷酶的能力.以昆布多糖得率为评价指标,通过正交试验确定复合酶配比,采用响应面法评价酶解时间、pH、液料比和温度对昆布多糖得率的影响.采用体外酶抑制实验测定昆布多糖对α-葡萄糖苷酶的抑制活性.结果表明,复合酶最佳添加量为纤维素酶 100 mg、果胶酶 90 mg、木瓜蛋白酶 55 mg,最佳酶法提取工艺为酶解时间 1.8 h、酶解温度 49.4℃、pH6.1、液料比 59:1 mL/g,最佳工艺条件下昆布多糖预测得率 18.183%,实测多糖得率 18.19%±1.04%,其中性糖、酸性糖、蛋白质及硫酸根含量分别 52.72%、11.76%、2.66%、19.49%;在 1～5 mg/mL范围内其对α-葡萄糖苷酶的抑制作用随浓度增加而升高,最大抑制率为 79.04%±3.17%,IC50 为 1.443 mg/mL.复合酶法提取的昆布多糖得率高,其对α-葡萄糖苷酶具有明显的抑制作用.
Abstract
Multi-enzyme-assisted laminarin extraction parameters were optimized,and then the prepared laminarin was assessed for inhibition of α-glucosidase activity.The relative proportions of preparative enzymes were investigated to maximize laminarin yield using an orthogonal test design.The effect of enzymatic hydrolysis time,pH,ratio of liquid to material and temperature on laminarin yield were determined using response surface methodology(RSM).Finally,laminarin inhibition of α-glucosidase activity was assessed using an in vitro enzyme inhibition test.Results revealed that the optimal laminarin yield was obtained using a ratio by mass of 10:9:5.5 of cellulase,pectinase and papain,respectively,and an enzymatic hydrolysis time,temperature,pH and ratio of liquid to material of 1.8 h,49.4℃,6.1 and 59:1 mL/g,respectively.The predicted yield of laminarin under optimal conditions was 18.183%,which resembled the actual laminarin yield of 18.19%±1.04%.Its neutral sugar,acid sugar,protein and sulphate contents were 52.72%,11.76%,2.66%and 19.49%,respectively.As laminarin concentration increased from 1 mg/mL to 5 mg/mL,its α-glucosidase inhibition activity increased,with highest inhibition of 79.04%±3.17%and IC50 value of 1.443 mg/mL.This multi-enzyme-assisted extraction procedure generated a high yield of laminarin that exerted clearly demonstrable inhibition ofα-glucosidase activity.
关键词
昆布多糖/复合酶/提取工艺/α-葡萄糖苷酶活性Key words
laminarin/complex enzyme/extraction process/α-glucosidase activity引用本文复制引用
基金项目
中央引导地方科技发展资金(202002050JC)
技术创新引导医药健康产业发展专项(20210401058YY)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据