铁皮石斛多糖的低共熔溶剂提取工艺优化
Optimization of Deep Eutectic Solvent Extraction Process of Polysaccharides from Dendrobium officinale
冯思敏 1廖伟先 1潘杰峰 2余佳浩 1陈碧莲 3邵平1
作者信息
- 1. 浙江工业大学食品科学与工程学院,浙江杭州 310014
- 2. 浙江工业大学化学工程学院,浙江杭州 310014
- 3. 浙江省食品药品检验研究院,浙江杭州 310052
- 折叠
摘要
为提高铁皮石斛的综合利用率,建立一种绿色高效的铁皮石斛多糖提取方法.本研究以铁皮石斛多糖提取率为指标,通过单因素考察了低共熔溶剂浓度、提取温度及液料比对铁皮石斛多糖提取率的影响,采用响应面设计优化铁皮石斛多糖的提取工艺,并对纯化后的多糖进行结构分析.结果表明:响应面优化后得到最佳工艺为低共熔溶剂(deep eutectic solvent,DES)浓度 40%、提取温度 80℃、液料比 110:1(mL/g),在此条件下实际提取率为 33.2%±0.28%,与预测值 33.5%接近,多糖的纯度为 56.95%±1.2%.多糖经阴离子交换柱及葡聚糖凝胶柱纯化后,纯度可达 90.8%,其单糖主要由葡萄糖和甘露糖构成,质量比约为 43:37,此外还含有少量的木糖、鼠李糖、核糖等,结构中同时含有α糖苷键和β糖苷键.本研究提供了一种低共熔溶剂提取铁皮石斛多糖的高效绿色提取方案,具有多糖提取率高及绿色的特点,为后续铁皮石斛多糖的开发提供了借鉴.
Abstract
In order to increase the comprehensive utilization of Dendrobium officinale,a green and efficient method for extracting of Dendrobium officinale polysaccharides(DOPs)was established.This research used the extraction rate of DOPs as the indicator for the effects of three factors.These included the concentration of deep eutectic solvent(DES),extraction temperature,and liquid-to-material ratio on the efficacy of polysaccharides from Dendrobium officinale,using single-factor tests.Response surface methodology(RSM)was employed to optimize the best extraction process of DOPs and the structure of the purified polysaccharides was analyzed.The experimental results showed that the optimal process obtained after RSM was a 40%concentration of DES,an extraction temperature of 80℃,and a liquid-to-material ratio of 110:1(mL/g).Under these conditions,the actual extraction rate was 33.2%±0.28%,which was close to the predicted value of 33.5%,and the purity of polysaccharide was 56.95%±1.2%.After purification by anion-exchange column and glucose gel column,the purity could reach 90.8%,and its monosaccharides were mainly composed of glucose and mannose,with a mass ratio of 43:37.It also contained a small amount of xylose,rhamnose,and ribose,and the structure contained α-glycosidic and β-glycosidic bonds.The present research provides an efficient and green extraction scheme for extracting DOPS with DES,which has the characteristics of high polysaccharide extraction rate and greenness,providing a reference for the subsequent development of DOPs.
关键词
铁皮石斛多糖/低共熔溶剂/提取工艺/响应面/结构Key words
Dendrobium officinale polysaccharides/deep eutectic solvent/extraction process/response surface/structure引用本文复制引用
基金项目
国家食品营养与安全重点研发计划专项(2023YFF1104100)
浙江省重点研究发展计划项目(2021C02019)
浙江省自然科学基金项目(LGD22C200001)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据