摘要
为了探讨不同贮藏温度对离土浙贝母贮藏品质以及酶活性的影响,以浙贝母为试材,将浙贝母分别置于0、5、10和 20℃四个温度下贮藏 90 d,研究浙贝母在不同贮藏温度下的失重、感官品质、色泽、硬度、总多糖、生物碱、丙二醛(malondialdehyde,MDA)含量、多酚氧化酶(polyphenol oxidase,PPO)、过氧化物酶(peroxidase,POD)以及超氧化物歧化酶(superoxide dismutase,SOD)活性的变化.结果表明:贮藏 90 d,0℃贮藏的浙贝母失重率仅为 7.17%,显著低于 5、10和 20℃(9.92%、19.77%和 30.28%)(P<0.05);与 5、10和20℃相比,0℃贮藏更好地保持浙贝母的色泽、硬度、总多糖含量以及总生物碱含量;与5、10和20℃相比,0℃贮藏有效抑制了MDA含量上升,延缓了浙贝母鳞茎的衰老进程;同时 0℃贮藏有效抑制了多酚氧化酶和过氧化物酶活力,而且有利于SOD维持较高的活性.与 5、10和 20℃相比,0℃贮藏的浙贝母品质保持得最好.本研究为浙贝母离土贮藏提供了方法参考及理论依据.
Abstract
In order to investigate the effects of different storage temperatures on the storage quality and enzyme activities of Fritillaria thunbergii Miq from the soil,which were stored at 0,5,10 and 20℃for 90 d.The changes in weight loss,sensory quality,color,hardness,total polysaccharides,alkaloids,malondialdehyde(MDA)contents,polyphenol oxidase(PPO),peroxidase(POD)and superoxide dismutase(SOD)activities of Fritillaria thunbergii Miq were explored at different storage temperatures.The results showed that the weight loss rate of Fritillaria thunbergii Miqat0℃for90d was only 7.17%,which was significantly lower than that at 5,10 and 20℃(9.92%,19.77%and 30.28%)(P<0.05).The color,hardness,total polysaccharide content and total alkaloid content of Fritillaria thunbergii Miq were better maintained at 0℃storage compared with 5,10 and 20℃.The increment of MDA content was effectively inhibited at 0℃storage,thus delaying the aging process of Fritillaria thunbergii Miq bulb.Meanwhile,the activities of PPO and POD were effectively inhibited and the maintenance of higher SOD activity at 0℃storage.Compared with 5,10 and 20℃,the quality of Fritillaria thunbergii Miq was best maintained in 0℃storage.Thus,this study provides a methodological reference and a theoretical basis for the off-soil storage of Fritillaria thunbergii Miq.
基金项目
宁波市公益类科技计划项目(202002N3087)
国家科技期刊平台
NSTL
维普
万方数据