维生素K2单独及与钙剂联合使用对斑马鱼骨骼健康的改善作用及机制研究
Effect of Vitamin K2 Alone and in Combination with Calcium on the Bone Health Improvement and Mechanism in Zebrafish Model
孙怡 1夏洪志 1牛堃 1李江波 1朱宇雷 1李古月 1尹忠燕1
作者信息
- 1. 南通励成生物工程有限公司,江苏南通 226010
- 折叠
摘要
目的:研究维生素K2 单独使用及与钙剂联合使用对斑马鱼骨骼健康改善作用及机制.方法:采用转基因Tg系健康斑马鱼作为实验动物,利用泼尼松龙诱导骨质疏松斑马鱼模型,显微镜下观察并拍照,以斑马鱼成骨细胞荧光面积和荧光密度为指标,评价药物处理后斑马鱼骨骼发育情况,并采用Real-time qPCR检测相关基因表达变化.结果:在 5～20 μg/mL的实验安全剂量范围内,维生素K2 及与D3 和钙剂联合使用,均能显著改善由泼尼松龙诱导的斑马鱼成骨细胞荧光面积和荧光密度的降低(P<0.05);维生素K2 通过促进成骨细胞生成相关的alp、bmp2a和bmp4基因的表达,抑制破骨细胞生成相关的opg、rank1、trap、ctsk基因的表达以及抑制细胞外基质中骨钙化相关基因vdrb、sparc、colla2表达从而发挥功效.结论:维生素K2 通过促进成骨细胞生成相关基因的表达,抑制破骨细胞和细胞外基质中骨钙化相关基因的表达,发挥改善斑马鱼骨骼健康的作用,其与钙剂联合给药,可提升其改善骨骼健康能力.
Abstract
Objective:To investigate the bone health improvement effects and mechanism of vitamin K2 alone and in combination with calcium on osteoporotic zebrafish model.Methods:Healthy zebrafish with transgenic Tg line were used as experimental animals.The model of osteoporosis zebrafish was induced by prednisone.The fluorescence area and density of zebrafish osteoblasts were used as indicators to evaluate the bone development of zebrafish which were observed and photographed under microscope.Furthermore,the expression changes of related genes were detected by Real-time qPCR.Results:Vitamin K2 alone and in combination with D3 and calcium chloride could significantly improve the reduction of fluorescence area and fluorescence density of zebrafish osteoblasts induced by prednisolone in the experimental safe dose range of 5～20 μg/mL(P<0.05).Vitamin K2 played anti-osteoporosis activity by promoting the expression of alp,bmp2a and bmp4 genes related to osteoblast,inhibiting the expression of opg,rank1,trap and ctsk genes related to osteoclast,and inhibiting the expression of vdrb,sparc and colla2 genes related to bone extracellular matrix.Conclusion:Vitamin K2 could improve the bone health of zebrafish by promoting the expression of genes related to osteoblast formation and inhibit the expression of genes related to osteoclasts and bone calcification in extracellular matrix.The combined administration of vitamin K2 with D3 and calcium chloride could enhance the ability to improve bone health.
关键词
维生素K2/斑马鱼/骨质疏松/作用机制Key words
vitamin K2/zebrafish/osteoporosis/mechanism引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据