Objective:To research the therapeutic effects and mechanisms of ligustilide(Lig)in improving dextran sodium sulfate(DSS)-induced ulcerative colitis(UC)in mice.Methods:Intervention of lig was delivered in sixty male SPF C57 BL/6 mice and the mice were differentiated in six groups according to the dose of lig as:Control group,DSS group,positive control group(treated with sulfasalazine,SASP),low-dose lig group,medium-dose lig group,and high-dose lig group.The UC in DSS mice was induced by a 3%DSS solution through oral administration for 7 days,in the meanwhile,the low,medium,and high-dose lig groups were received gavage feeding of lig and oral administration of 3%DSS solution.Before and after the intervention,the body weight,colon length,and the disease activity index(DAI)score were collected for assessment of UC.Furthermore,the expression levels of TLR4/NF-κB proteins in colon and serum TNF-α,IL-6,and IL-1β were quantitatively measured using enzyme-linked immunosorbent(ELISA)assays.Additionally,histological exam-ination was performed to investigate the effects and mechanisms of lig in improving UC in mice by hematoxylin-eosin staining.Results:Compared to the control group,significantly reduced body weight(P<0.05),shorter colon length(P<0.05),increased DAI score(P<0.05).And there was a large amount of inflammatory infiltration in the intestine,the expression level of TNF-α,IL-6,and IL-1β in the colon significantly increased(P<0.05).Compared with DSS group,significant suppression of the TLR4/NF-κB signaling pathway in the intestinal tissue,serum TNF-α,IL-6,and IL-1β expression(P<0.05)were observed in the medium and high-dose lig groups,which indicated a significant improvement in intestinal damage.Conclusion:Lig would be able to effectively improve DSS-induced UC in mice.Moreover,the results showed that the mechanism of lig improving DSS-induced UC was possibly involved with the inhibition of the NF-κB signaling pathway.
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