Glucansucrase(EC.2.4.5.1)is a class of α-glucosyltransferases,mainly produced by lactic acid bacteria such as Leuconostoc,Streptococcus and Lactobacillus.The structure and catalytic mechanism of glucansucrase are diverse,and it is an important tool enzyme for the biosynthesis of exopolysaccharide.This article mainly reviews the source,classification,structure and reaction mechanism of glucansucrase and the effects of medium composition,culture conditions on the production of glucansucrase,with a focus on the optimization methods,separation and purification processes,and enzym-atic properties of glucansucrase,and prospects for its development trend.This article aims to provide reference for the research of glucansucrase in related fields.
关键词
葡聚糖蔗糖酶/结构/催化机理/优化/分离纯化/应用
Key words
glucansucrase/structure/catalytic mechanism/optimization/isolation and purification/application