Objective:In this work,the optimal preparation process parameters of selenium-curcumin nanoparticles(SeNPs@Cur)were explored and its protective effect on alcoholic liver injury was evaluated.Methods:On the basis of single factor experiment,with particle size as the index,curcumin dosage,molar ratio of VC to Na2SeO3,reaction time and reaction temperature were selected as influence factors.The process parameters of SeNPs@Cur were optimized by response surface methodology,and its structure was characterized by scanning electron microscope(SEM),fourier transform infrared spectrometer(FT-IR)and energy dispersive spectrometer(EDS).The protective effect of SeNPs@Cur on ethanol-induced LO2 cell injury was investigated by MTT method.Results:The optimal conditions of SeNPs@Cur were list as follows:Curcumin dosage of 5.92 mg/mL,molar ratio of VC to Na2SeO3 of 10.39:1,the reaction time of 1.98 h,and the reaction temperature of 34.00℃.Under these conditions,the SeNPs@Cur with a minimum size of(100.79±3.46)nm was obtained.The loading rate of curcumin and the selenium content in the sample were 12.80%±0.80%and 23.32%±0.07%,respectively.Further structural identification showed that the prepared SeNPs@Cur was dispersed spherical nanoparticles.Moreover,compared with the model group,after treatments with different dosages of SeNPs@Cur,the cell viabilities increased from 58.06%±0.43%to 71.43%±1.39%,77.33%±3.54%,and 85.41%±4.61%,respectively,indicating that SeNPs@Cur had a protective effect on LO2 cells damaged by ethanol in a concentration-dependent manner.Conclusion:The SeNPs@Cur with anti-alcoholic liver injury effect was successfully obtained,which provided new technical means and theoretical reference data for formulation improvement and application of curcumin.
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