食品工业科技2024,Vol.45Issue(5) :412-425.DOI:10.13386/j.issn1002-0306.2023050216

基于核酸扩增-侧流层析试纸的食源性致病菌快速检测研究进展

Advances in the Rapid Detection of Foodborne Pathogens by Nucleic Acid Amplification Lateral Flow Assays

贠紫光 魏勇 张建 崔双双 李灿 孙凤霞
食品工业科技2024,Vol.45Issue(5) :412-425.DOI:10.13386/j.issn1002-0306.2023050216

基于核酸扩增-侧流层析试纸的食源性致病菌快速检测研究进展

Advances in the Rapid Detection of Foodborne Pathogens by Nucleic Acid Amplification Lateral Flow Assays

贠紫光 1魏勇 2张建 1崔双双 1李灿 1孙凤霞3
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作者信息

  • 1. 农业农村部特色农产品加工与质量安全控制重点实验室(部省共建),石河子大学食品学院,新疆石河子 832000;食品营养与安全控制兵团重点实验室,石河子大学食品学院,新疆石河子 832000
  • 2. 新疆天润乳业股份有限公司,新疆乌鲁木齐 830063
  • 3. 农业农村部特色农产品加工与质量安全控制重点实验室(部省共建),石河子大学食品学院,新疆石河子 832000;食品营养与安全控制兵团重点实验室,石河子大学食品学院,新疆石河子 832000;新疆天润乳业股份有限公司,新疆乌鲁木齐 830063
  • 折叠

摘要

食源性致病菌严重危害食品安全.传统的致病菌检测方法费时耗力,难以应对现代食品安全检测快速、简便的需求.分子生物学检测方法,如聚合酶链式反应法、等温核酸扩增法以及基于规律成簇间隔短回文重复序列的核酸检测技术等,已被广泛应用于食源性致病菌检测,为保障食品安全发挥了重要作用.近年来,联用核酸扩增检测技术和免疫层析技术成为食源性致病菌快速检测的研究热点.本文总结了核酸扩增和侧流层析试纸联用快速检测食源性致病菌的研究进展,重点讨论了其优缺点,并展望其未来发展方向,以期为食源性致病菌的检测和防控提供参考.

Abstract

Foodborne pathogens remain a serious threat to food safety,while traditional assays are time-consuming and labor-intensive,and difficult to match with the rapid and simple requirements of modern food safety detection.Molecular biology-based assays,such as polymerase chain reaction,isothermal nucleic acid amplification,and clustered regularly interspaced short palindromic repeats-based nucleic acid detection technology,have been widely employed in the detection of foodborne pathogens,and play an important role in ensuring food safety.In recent years,the combination of nucleic acid amplification detection technology and immunochromatography technology has become a hotspot for rapidly detecting foodborne pathogens.Here,the research progress in the rapid detection of foodborne pathogens are summarized by combining nucleic acid amplification and lateral flow immunoassays,highlight their advantages and disadvantages,and provide a comprehensive outlook on this field.This review can provide a reference for detecting,preventing,and controlling foodborne pathogens.

关键词

食源性致病菌/核酸扩增/侧流层析试纸/快速检测

Key words

foodborne pathogens/nucleic acid amplification/lateral flow strip/rapid detection

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基金项目

石河子大学高层次人才项目(RCZK201920)

石河子大学科技项目(ZZZC201908A)

兵团十二师科技项目(SR2020001)

兵团十二师科技项目(SR202101)

兵团科技项目(2022CB014-01)

出版年

2024
食品工业科技
北京一轻研究院

食品工业科技

CSTPCD北大核心
影响因子:0.842
ISSN:1002-0306
参考文献量26
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