食品工业科技2024,Vol.45Issue(5) :426-437.DOI:10.13386/j.issn1002-0306.2023060053

食源性致病菌快速检测技术及其标准化应用研究进展

Research Progress on Rapid Detection Technology and Standardized Application of Foodborne Pathogens

吴鹏 孙雅和 朱旭丽 周树华 张成云
食品工业科技2024,Vol.45Issue(5) :426-437.DOI:10.13386/j.issn1002-0306.2023060053

食源性致病菌快速检测技术及其标准化应用研究进展

Research Progress on Rapid Detection Technology and Standardized Application of Foodborne Pathogens

吴鹏 1孙雅和 1朱旭丽 1周树华 1张成云2
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作者信息

  • 1. 浙江省标准化研究院,金砖国家标准化(浙江)研究中心,之江标准化智库,国家市场监管数字化研究与应用技术创新中心,浙江杭州 310007
  • 2. 文成县食品药品综合检测中心,浙江温州 325399
  • 折叠

摘要

随着新型食品安全检测技术的快速发展,食源性致病菌快速检测技术改善了传统检测方法周期长、灵敏度低等缺陷,对于保障民众生命健康和经济社会发展起到重要作用,而标准化则是推动快速检测技术应用推广的关键和前提.本文系统介绍了生理生化检测技术、免疫学检测技术、分子检测技术等目前使用较多的食源性致病菌快速检测方法,总结了各类不同方法的技术原理、研究进展及优缺点,并从标准化角度进一步介绍了国内外快速检测技术的标准现状以及应用实践情况.新型快速检测技术具备灵敏、快速、特异性强的优势,但也存在一定的缺陷,如免疫检测技术抗体前处理较为麻烦,生理生化检测技术有污染菌混淆问题,分子检测技术有一定假阳性等.此外,大部分检测方法仍属于非法定方法,缺乏统一的判定标准,国内外相关标准体系仍不完善,制约了快速检测技术的标准化推广应用.基于此,本文从标准化角度提出了食源性致病菌快速检测技术未来发展的建议,以期为后续食品安全相关技术的研究与标准化建设提供参考.

Abstract

In recent years,with the rapid development of new food safety testing technologies,rapid detection of foodborne pathogens has improved traditional detection methods that suffer from long cycles and low sensitivity,thus playing an important role in safeguarding public health and promoting economic and social development.Standardization is the key to the promotion and application of rapid detection technologies.This article systematically introduces commonly used rapid detection methods for foodborne pathogens,including physiological and biochemical detection,immunological detection,and molecular detection.The principles,research progress,advantages,and disadvantages of various methods are summarized.What's more,domestic and abroad standardization status and application of rapid detection technologies are further introduced from a standardization perspective.The new rapid detection technology has the advantages of sensitivity,speed,and strong specificity,but there are also certain shortcomings,such as the cumbersome pre-treatment of antibodies in immune detection technology,the confusion of contaminated bacteria in physiological and biochemical detection technology,and certain false positives in molecular detection technology.In addition,most detection methods are still illegal and lack unified judgment standards.The relevant standard systems at home and abroad are still incomplete,which restricts the standardized promotion and application of rapid detection technology.Therefore,this article proposes suggestions for the future development of rapid detection technology for foodborne pathogens from the perspective of standardization,in order to provide reference for the research and standardization construction of food safety-related technologies in the future.

关键词

食源性致病菌/快速检测技术/标准化应用

Key words

foodborne pathogen/rapid detection technology/standardized application

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基金项目

浙江省市场监督管理局科技计划项目(ZC2023020)

出版年

2024
食品工业科技
北京一轻研究院

食品工业科技

CSTPCD北大核心
影响因子:0.842
ISSN:1002-0306
被引量2
参考文献量17
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