黑果枸杞花色苷的提取、纯化及降解动力学研究
Study on Extraction,Purification and Degradation Kinetics of Anthocyanins from Lycium ruthenicum
连敏 1高艺玮 1年新 1王梦泽1
作者信息
- 1. 宁夏大学食品科学与工程学院,宁夏银川 750021
- 折叠
摘要
以花色苷提取量为主要考察指标,通过单因素和正交试验优化冻干黑果枸杞花色苷提取工艺,并在此条件下研究花色苷纯化工艺及其降解动力学,探讨不同温度、pH下花色苷提取量的变化.结果表明,提取最佳工艺条件为:料液比 1:25(g:mL)、乙醇浓度 60%、pH4、提取时间 2h,此条件下花色苷提取量达 36.507±0.325 mg/g.研究显示AB-8大孔树脂纯化黑果枸杞花色苷效果最好,对花色苷吸附量和解吸量的影响效果最佳,其最佳条件为:上样液浓度 200 mg/100 g,解吸乙醇浓度 80%,上样流速 2 mL/min,洗脱流速 2 mL/min,上样体积为 5 BV,纯化率为 90.02%.降解动力学研究结果表明:相同pH下,随着温度升高花色苷的降解速率增大、半衰期减少;此外,花色苷在酸性环境中较稳定,在碱性环境下易降解,当pH为 3,50℃时花色苷稳定性最好,t1/2 最大为38.5 h,Ea最大为 41.89 kJ/mol.因此黑果枸杞花色苷提取量与其提取、纯化及温度、pH的工艺条件密切相关,本研究为黑果枸杞花色苷的提取及饮品开发提供技术支撑.
Abstract
In this study,taking the amount of anthocyanin extraction as the main index,the extraction process of lyophilized Lycium ruthenicum anthocyanin was optimized by one-way and orthogonal tests,and the purification process of anthocyanin and its degradation kinetics were studied under these conditions,to explore the changes of the extraction amount of anthocyanin under different temperatures and pH.The results showed that the optimal conditions for the extraction process:The material-liquid ratio was 1:25(g:mL),the ethanol concentration was 60%,the pH was 4,and the extraction time was 2 h,which resulted in a purification rate of 90.02%.The amount of anthocyanosides extracted under these conditions amounted to 36.507±0.325 mg/g.In addition,the study showed that AB-8 macroporous resin was the most effective in purifying anthocyanin from Lycium ruthenicum,and it had the best effect on the adsorption and desorption of anthocyanin.The optimal conditions were:The concentration of the sample solution was 200 mg/100 g,the concentration of desorbed ethanol was 80%,the flow rate of the sample was 2 mL/min,the flow rate of elution was 2 mL/min,and the volume of the sample was 5 BV.The results of the degradation kinetics study showed that the degradation rate of anthocyanin increased with increasing temperature and the half-life decreased at the same pH.Moreover,anthocyanosides were more stable in acidic environment and easily degraded in alkaline environment,and the stability of anthocyanosides was the best with the maximum of t1/2 of 38.5 h and the maximum of Ea of 41.89 kJ/mol at pH3 and 50℃.Therefore,the content of Lycium ruthenicum anthocyanin is closely related to its extraction,purification and stability of process conditions,this study provides technical support for the extraction of wolfberry anthocyanin and the development of beverages.
关键词
黑果枸杞/花色苷/提取/纯化/降解动力学Key words
Lycium ruthenicum/anthocyanins/extraction/purification/degradation kinetics引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据