响应面法优化相思藤黄酮提取工艺及其体外抗氧化活性分析
Optimization of Extraction Process by Response Surface Method and Analysis of Antioxidant Activityin Vitroof Total Flavonoids from Abrus precatorius Linn
龚受基 1覃媚 2戴梓茹 2蒋红明 3郭德军3
作者信息
- 1. 北部湾大学食品工程学院,广西钦州 535011;广西高校北部湾海产品高值化利用与预制食品重点实验室,广西钦州 535011;钦州市特色果蔬发酵重点实验室,广西钦州 535011
- 2. 北部湾大学食品工程学院,广西钦州 535011;广西高校北部湾海产品高值化利用与预制食品重点实验室,广西钦州 535011
- 3. 北部湾大学食品工程学院,广西钦州 535011;钦州市特色果蔬发酵重点实验室,广西钦州 535011
- 折叠
摘要
为优化相思藤黄酮的提取工艺,探讨其抗氧化活性,以乙醇浓度、料液比、超声能量、超声时间为影响因素,以黄酮得率为考核指标,采用单因素和Box-Behnken法优化相思藤黄酮提取工艺,并通过检测相思藤黄酮对DPPH、ABTS+自由基的清除能力和对亚铁离子的还原力探讨其抗氧化能力.结果表明,在料液比(W/V)为1:50时,相思藤黄酮的最佳提取工艺为乙醇浓度 82%、超声能量 507 J、超声时间 42 min,此时相思藤黄酮得率为 0.696%.相思藤黄酮能有效清除DPPH、ABTS+自由基,清除半数有效浓度EC50 分别为 0.028、0.009 mg/mL,清除能力优于水溶性维生素E(Trolox),且在一定浓度范围内清除能力随着质量浓度的增加而增强;相思藤黄酮浓度为 5 mg/mL时对铁离子的还原力为 2.42 mmol/L,弱于Trolox.综上,应用超声提取技术可有效提取相思藤黄酮,提取的黄酮具有良好的抗氧化活性.
Abstract
The objective of this study was to optimize the extraction process of flavonoids from Abrus precatorius Linn,and explored its antioxidant activity.Ethanol volume fraction,solid-liquid ratio,ultrasonic energy and ultrasonic extracting time as variables,the yield of flavonoids as evaluation index,single factor tests and Box-Behnken method were used to optimize the extraction process.The scavenging ability on DPPH,ABTS+ free radicals,and the reducing power of flavonoids from Abrus precatorius Linn were also detected to estimate the antioxidant activities.Results showed that,when solid-liquid ratio reached 1:50,the optimum extraction process(0.696%,highest yield)could be achieved under condition of ethanol volume fraction 82%,ultrasonic energy 507 J and ultrasonic extracting time 42 min.Half effective concentration being EC500.028 and 0.009 mg/mL respectively.Abrus precatorius Linn flavonoids were able to scavenge DPPH and ABTS+ free radicals effectively,and its ability risen as mass concentration increases,meaning it is superior to Trolox.When flavonoid concentration was set at 5 mg/mL,the reducing power on iron ion reached 2.42 mmol/L,which was weaker than Trolox.To sum up,ultrasonic extraction technology can be effectively employed to the extraction of Abrus precatorius Linn flavonoids,resulting in better antioxidant activity.
关键词
相思藤/黄酮/提取工艺/抗氧化活性Key words
Abrus precatorius Linn/flavonoids/extraction technology/antioxidant activity引用本文复制引用
基金项目
广西壮族自治区教育厅:广西高校中青年教师科研基础能力提升项目(2022KY0425)
钦州市科技攻关(202014820)
出版年
2024
国家科技期刊平台
NSTL
万方数据