人参不定根总皂苷的提取工艺优化及其抗氧化与抗疲劳作用
Process Optimization of Total Saponins from Adventitious Roots of Ginseng and Their Antioxidant and Anti-fatigue Effects
邢艺缤 1王馨悦 1王慕尧 1齐欣 1崔承弼2
作者信息
- 1. 延边大学农学院,吉林延吉 133000
- 2. 延边大学农学院,吉林延吉 133000;延边大学药学院,吉林延吉 133000
- 折叠
摘要
目的:探究人参不定根总皂苷(ginseng adventitious roots total saponins,GARS)的最佳提取工艺及抗氧化和抗疲劳作用.方法:利用乙醇回流法提取GARS,通过正交试验考察乙醇浓度、料液比、提取温度、提取时间对GARS含量的影响;以 1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)、3-氧代-2-苯基-4,4,5,5-四甲基咪唑啉-1-氧(PTIO)、羟自由基(·OH)、超氧阴离子自由基(O2-·)清除能力及还原力作为测定抗氧化活性指标;通过测定小鼠力竭游泳和爬杆时间,测定肌/肝糖原、乳酸(LD)、尿素氮(BUN)含量及乳酸脱氢酶(LDH)活性来判定GARS抗疲劳能力.结果:通过正交试验得到最佳提取工艺条件为乙醇浓度 70%、料液比 1:30 g/mL、提取温度 70℃、提取时间 40 min,得到GARS含量为 107.85 mg/g;GARS对DPPH、ABTS+、PTIO、OH、O2-自由基都有一定程度清除作用,且具有还原性,其清除能力和还原性与浓度呈正比;GARS能够显著延长小鼠力竭游泳和爬杆时间(P<0.05),显著增加肌/肝糖原含量(P<0.05),降低LD、BUN含量,提高LDH活性,并与其浓度呈正比.结论:GARS提取的最优条件为乙醇浓度 70%、料液比1:30 g/mL、提取温度 70℃、提取时间 40 min,可为GARS产业化生产提供依据,且GARS具有一定的抗氧化和抗疲劳作用,可为明确人参不定根营养成分和活性物质提供理论支持,为开发人参不定根药食同源的相关产品提供技术参考.
Abstract
Objective:To investigate the optimal extraction process and antioxidant and anti-fatigue activities of ginseng adventitious roots total saponins(GARS).Methods:GARS was extracted using ethanol reflux method,and the effects of different factors such as ethanol concentration,solvent-to-solid ratio,extraction temperature,and extraction time on the content of GARS were studied by orthogonal test.The antioxidant and anti-fatigue activities of GARS were determined by measuring the scavenging abilities of DPPH,ABTS+,PTIO,·OH,and O2-·,as well as the reducing power.The effects of GARS on the swimming and climbing times of mice,the contents of muscle/gallbladder glycogen,lactate(LD),and urea nitrogen(BUN)were determined to evaluate its anti-fatigue ability.Results:The optimal extraction conditions were ethanol concentration of 70%,solvent-to-solid ratio of 1:30 g/mL,extraction temperature of 70℃,and extraction time of 40 min,with a GARS content of 107.85 mg/g.GARS showed significant antioxidant and anti-fatigue activities,with its antioxidant and reducing power being positively correlated with its concentration.GARS significantly prolonged the swimming and climbing times of mice(P<0.05),increased the contents of muscle/gallbladder glycogen(P<0.05),decreased the levels of LD and BUN,and increased the activity of lactate dehydrogenase(LDH),indicating its anti-fatigue ability.Conclusion:The optimal extraction process of GARS was ethanol reflux method with ethanol concentration of 70%,solvent-to-solid ratio of 1:30 g/mL,extraction temperature of 70℃,and extraction time of 40 min.GARS showed significant antioxidant and anti-fatigue activities,which can support the development of ginseng adventitious roots as a food or health supplement ingredient.
关键词
人参不定根/总皂苷提取物/工艺优化/抗氧化/抗疲劳Key words
ginseng adventitious roots/total saponins extract/process optimization/antioxidation/antifatigue引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据