摘要
为探究银杏内酯B(ginkgolide B,GB)对肥胖小鼠的干预作用,首先采用高脂膳食诱导构建C57BL/6J小鼠肥胖模型,随后将肥胖小鼠按体重随机分为 4组,模型组(PG)、GB低剂量组(GBL)、GB中剂量组(GBM)和GB高剂量组(GBH),进行为期 8周的干预实验,测定各组小鼠肥胖相关指标,如体重变化、脏器脂肪系数、血脂指标等.GB干预后,GBM和 GBH组小鼠的体重增长明显小于 PG组(P<0.05),同时,GBH组的小鼠脏器脂肪系数均显著降低(P<0.05).另外,GBM和GBH组小鼠的血脂指标总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HLD-C)均得到显著(P<0.05)改善,尤其是GBH组小鼠;最后,组织病理学结果显示,GBM和GBH组小鼠肝脏脂肪沉积明显减轻.进一步实时荧光定量聚合酶链式反应(Quantitative Real-time Polymerase Chain Reaction,qRT-PCR)结果显示,和PG组相比,GBM和GBH组小鼠肝脏中过氧化物酶体增殖物激活受体 γ(peroxisome proliferator-activated receptorγ,PPARγ)的mRNA表达水平显著降低(P<0.05),而解偶联蛋白 2(Uncoupling protein 2,UCP-2)的mRNA水平显著增加(P<0.05).本研究发现中高剂量的GB可以有效改善小鼠的肥胖,并且这种改善作用可能和PPARγ-UCP-2信号途径相关.
Abstract
This study aims to explore the effect of ginkgolide B(GB)on obese mice.An obese C57BL/6J mice model was induced by high-fat diet firstly,and then the obese mice were divided into 4 groups according to body weight randomly:Positive model group(PG),GB low dose group(GBL),GB medium dose group(GBM)and GB high dose group(GBH).After 8-week intervention,the obesity-related indexes(weight change,visceral fat coefficient and serum indexes)of mice in each group were measured.Results showed that the weight gain of mice in GBM and GBH were significantly decreased than in PG(P<0.05),and the visceral fat coefficient of mice in GBH group was also reduced significantly(P<0.05)after GB treatment.Meanwhile,the serum indexes(TG,TC,LDL-C and HDL-C)of mice in GBM and GBH were obviously improved,especially in GBH.Besides,histopathologic findings showed that liver fat deposition was apparently reduced in GBM and GBH.Furthermore,qRT-PCR results showed that the mRNA expression level of PPARγ in liver of mice in GBM and GBH was significantly decreased(P<0.05)in comparison with PG,while the mRNA expression level of UCP-2 was significantly increased(P<0.05).In summary,this study demonstrated that GB of medium and high doses improved obesity in mice,and this ameliorating effect might be related to PPARγ-UCP-2 signaling pathway.
基金项目
江苏高校自然科学研究项目(21KJA350001)
国家科技期刊平台
NSTL
万方数据