摘要
为了探究不同生长时期酸枣果肉中多糖含量、相对分子质量、单糖组成及抗氧化活性变化规律,以野生型和种植型酸枣为研究对象,分别采用苯酚-硫酸比色法、高效凝胶色谱法、高效离子色谱法和DPPH自由基清除实验研究其不同生长时期的果肉多糖含量、相对分子质量、单糖组成和自由基清除能力.研究发现野生型和种植型的酸枣果肉中多糖含量均表现出先下降后上升的趋势;两种类型的酸枣果肉多糖的相对分子质量均随着酸枣的发育逐渐降低;两种类型酸枣果实发育前期果肉多糖中单糖鼠李糖含量最高可达 46.14 mg/g,半乳糖次之可达33.10 mg/g;果实发育后期,两种类型的酸枣果肉多糖中单糖含量最高的为阿拉伯糖可达 60.30 mg/g,半乳糖醛酸次之可达 45.02 mg/g;两种类型酸枣果肉多糖的DPPH自由基清除能力变化趋势与其果肉中多糖含量变化趋势基本相同;种植型酸枣与野生型酸枣在多糖含量、多糖相对分子质量、单糖组成和自由基清除能力方面均表现为相同的趋势,且均属于还原糖积累类型果实.研究结果为酸枣果肉资源进一步开发与利用提供参考.
Abstract
For the purpose of exploring the polysaccharide content,relative molecular mass,monosaccharide composition,as well as antioxidant activity in Ziziphus jujuba flesh polysaccharide(ZJFP)at distinct growth stages.The content,relative molecular weight,monosaccharide composition and free radical scavenging ability of ZJFP in wild-type and plant-type at different growth stages were studied by phenol-sulfuric acid assay,high performance gel chromatography,high performance ion chromatography,and DPPH free radical scavenging experiment.Moreover,the results determined that the polysaccharide content of ZJFP in both wild and plant types demonstrated a trend of first decreasing and consequently rising.Furthermore,the relative molecular mass of the two types of ZJFP gradually decreased with the development of the Ziziphus jujuba.During the initial stage of fruit development,the ZJFP exhibited the highest content of rhamnose,reaching 46.14 mg/g,followed by galactose up to 33.10 mg/g.Whereas,in the later stage of fruit development,the highest monosaccharide content in the two types of ZJFP was arabinose up to 60.30 mg/g,preceded by galacturonic acid up to 45.02 mg/g.Additionally,the trend of DPPH free radical scavenging capacity of ZJFP of the two types was identical to the trend of polysaccharide content in Ziziphus jujuba flesh.Plant-type and wild-type Ziziphus jujuba illustrated the identical trend regarding polysaccharide content,the relative molecular weight of polysaccharides,monosaccharide composition,as well as free radical scavenging ability,and both were of the type of fruit that reduces sugar accumulation.The research serve as a valuable reference for the future development and utilization of Ziziphus jujuba flesh resources.
基金项目
中央本级重大增减支项目(2060302)
咸阳市重大科技专项计划(2018k01-41)
陕西中医药大学博士科研启动金项目(17102032238)
国家科技期刊平台
NSTL
万方数据