复合酶法提取槐花多糖的工艺优化及其抗氧化活性
Optimization of Extraction Process of Polysaccharide from Sophora japonica by Compound Enzyme Method and Its Antioxidant Activity
任晓莉 1杨璐 1乔鹏 1缪奕锴 1杨懿昂 1代秋红 1张贤德1
作者信息
- 1. 太原工业学院环境与安全工程系,山西太原 030008
- 折叠
摘要
目的:采用复合酶法提取槐花多糖,对提取工艺进行优化,并评价其体外抗氧化活性.方法:通过单因素实验考察复合酶添加量、pH、复合酶比例和酶解时间对得率的影响,在单因素实验基础上,采用响应面法确定槐花多糖的最佳提取参数,并以VC为对照,通过测定槐花多糖对DPPH·和ABTS+·的清除率及总还原力,考察所提取的槐花多糖的抗氧化活性.结果:复合酶法提取槐花多糖的最佳提取参数为:复合酶添加量 23.8 mg/g,pH4.8,果胶酶与纤维素酶比例 0.912:1,该工艺下槐花多糖得率为 10.71%,所提取的槐花多糖对DPPH·和ABTS+·均表现出较好的清除能力,当槐花多糖溶液浓度为 2.8 mg/mL时,对DPPH·和ABTS+·的清除率分别达到同浓度下VC的94.19%和 99.79%,总还原力达到VC的 75.99%.结论:采用复合酶法能够有效提取槐花多糖,提高其抗氧化活性,为槐花多糖功能食品的开发提供了理论参考.
Abstract
Objective:Sophora japonica polysaccharides were extracted by compound enzyme method,and the extraction process was optimized.The antioxidant activity in vitro was evaluated.Methods:The effects of addition amount of compound enzyme,pH,proportion of compound enzyme and enzymatic hydrolysis time on the extraction yield were investigated by single factor experiment.On the basic of single factor experiment,response surface method was used to determine the optimal extraction parameters of Sophora japonica polysaccharide.Compared with VC,the antioxidant activity of Sophora japonica polysaccharides was investigated by measuring the scavenging rate of DPPH·and ABTS+·and the total reducing power.Results:The optimal extraction parameters of Sophora japonica polysaccharides were as follows:The addition amount of compound enzyme was 23.8 mg/g,pH4.8,and the ratio of pectinase to cellulase was 0.912:1.Under this process,the yield of Sophora japonica polysaccharides was 10.71%,and the extracted polysaccharide showed good scavenging ability for DPPH·and ABTS+·.When the concentration of the polysaccharide solution was 2.8 mg/mL,the scavenging rate of DPPH·and ABTS+·reached 94.19%and 99.79%of VC at the same concentration,respectively,and the total reducing power reached 75.99%of VC.Conclution:Sophora japonica polysaccharide could be effectively extracted by compound enzymatic method and its antioxidant activity could be improved,which provided a theoretical reference for the development of functional food of Sophora japonica polysaccharide.
关键词
槐花/多糖/复合酶/提取/抗氧化Key words
Sophora japonica/polysaccharides/compound enzyme/extraction/antioxidant引用本文复制引用
基金项目
大学生创新创业项目(GK2022131)
大学生创新创业项目(202314101022)
出版年
2024
国家科技期刊平台
NSTL
万方数据