黄花蒿精油抑菌、抗氧化及毒理学特性研究
Antibacterial,Antioxidant and Toxicological Properties of Artemisia annua Essential Oil
陈文丹 1白玉莹 2郭成虎 2柴玉宏 2王丰俊 3黄丛林 2刘华2
作者信息
- 1. 北京市农林科学院,北京 100089;中央储备粮宁陵直属库有限公司,河南商丘 476111;北京林业大学生物科学与技术学院,北京 100083
- 2. 北京市农林科学院,北京 100089
- 3. 北京林业大学生物科学与技术学院,北京 100083
- 折叠
摘要
采用水蒸气蒸馏法提取北京地区黄花蒿精油,通过气相色谱-质谱法(GC-MS)对黄花蒿精油的挥发性成分进行分析,通过测定黄花蒿精油对DPPH自由基、羟基自由基的清除率研究其体外抗氧化活性,采用琼脂扩散法评估抗菌活性,通过灌注小鼠不同剂量黄花蒿精油进行毒理学特性测定.结果表明:黄花蒿精油产率为1.04‰,GC-MS分析鉴定出 332种精油组分,其中 22个组分占总挥发物的 71.09%,相对含量较多的为蒿酮(19.34%)和(+)-α-蒎烯(6.10%);黄花蒿精油有较好的抗氧化性,对DPPH自由基、羟自由基的清除率与添加量呈量效关系,当其浓度为 10 mg/mL时,DPPH自由基、羟基自由基的清除率最大,分别为 40.03%和 92.97%;黄花蒿精油对金黄色葡萄球菌和大肠杆菌均有较明显的抑制作用,且对金黄色葡萄球菌的抑菌效果(12.67±0.29 mm)优于大肠杆菌的抑菌效果(9.27±0.25 mm);黄花蒿精油的LD50 为 7491 mg/kg,表明黄花蒿精油无毒性.该研究可为黄花蒿等蒿属植物的提取与利用提供理论参考.
Abstract
The essential oil of Artemisia annua grown in the Beijing area was extracted by hydrodistillation,and then its volatile components were analyzed by gas chromatography-mass spectrometry(GC-MS).The in vitro antioxidant activity of the Artemisia annua essential oil was determined by measuring the scavenging rate of DPPH and hydroxyl radicals,its antibacterial activity by using the agar diffusion method,and its toxicological properties by perfusing mice with different doses of the essential oil.The results showed that the extraction rate of the Artemisia annua essential oil was 1.04‰,with 332 essential oil components being identified by GC-MS analysis.Of these,22 components accounted for 71.09%of the total volatiles,with relatively high contents of artemisinone(19.34%)and(+)-α-pinene(6.10%).The Artemisia annua essential oil exhibited good scavenging activities of the DPPH and hydroxyl radicals in a dose-dependent relationship.The highest scavenging rates of the DPPH and hydroxyl radicals were 40.03%and 92.97%,respectively,at an essential oil concentration of 10 mg/mL.The Artemisia annua essential oil significantly inhibited the growth of both Staphylococcus aureus and Escherichia coli,with inhibitory zones of 12.67±0.29 mm and 9.27±0.25 mm,respectively.The LD50 value of the Artemisia annua essential oil was 7491 mg/kg,indicating that it was not toxic.This study can provide a theoretical reference for the extraction and use of Artemisia annua and other Artemisia plants.
关键词
黄花蒿精油/化学成分/抗氧化活性/抑菌性/毒理学特性Key words
Artemisia annua essential oil/chemical component/antioxidant activity/antimicrobial effect/toxicological properties引用本文复制引用
基金项目
北京市景观休闲农业创新团队(BAIC09-2023)
出版年
2024
国家科技期刊平台
NSTL
万方数据