西洋参花多糖闪式提取工艺优化及抗氧化活性研究
Optimization of Flash Extraction Process and Antioxidant Activity of American Ginseng Flower Polysaccharides
康忠禹 1赵大庆 1姚佳婧 2赵丽明 1黄宝泰 2刘莉 2齐滨2
作者信息
- 1. 长春中医药大学,吉林省人参科学研究院,吉林长春 130117
- 2. 长春中医药大学药学院,吉林长春 130117
- 折叠
摘要
目的:研究西洋参花多糖闪式提取的最佳工艺条件及抗氧化活性.方法:以西洋参花为原料,考察提取电压、液料比、提取时间对西洋参花多糖得率的影响,采用响应面法优化西洋参花多糖闪式提取工艺.通过测定西洋参花多糖对DPPH自由基和羟基自由基的清除作用及总还原力,考察西洋参花多糖的抗氧化活性.结果:通过实验得到西洋参花多糖的最佳提取工艺条件为:提取电压:130 V,液料比:30:1 mL/g,提取时间:100 s.在此条件下,西洋参花多糖得率为 11.12%±0.23%,与模型预测值相当;西洋参花多糖体外抗氧化显示其对DPPH自由基和羟基自由基清除率的IC50 值分别为 1.34、1.42 mg/mL,且具有一定还原力.表明西洋参花具有较强的抗氧化活性,且随着多糖浓度的增加,抗氧化活性不断增强.结论:本实验得到的提取工艺条件具有可行性,可用于西洋参花多糖的提取.西洋参花多糖具有较好的抗氧化活性.本研究可为西洋参花的开发利用提供理论依据.
Abstract
Objective:To study the optimal process conditions of flash extraction and antioxidant activity of American ginseng flower polysaccharides(AGFPs).Methods:American ginseng flower(AGF)as the raw material,the effects of extraction voltage,liquid-material ratio,and extraction time on the yield of AGFPs were explored.The flash extraction process of AGFPs was improved by response surface methodology.The scavenging effect on DPPH and hydroxy free radicals and total reduction capacity of AGFPs were determined to assess the antioxidant activity of AGFPs.Results:The optimal extraction conditions were determined as 130 V of extraction voltage,30:1 mL/g of liquid-material ratio,and 100 s of extraction time,resulting in an AGFPs yield of 11.12%±0.23%,which agreed with the model prediction.The AGFPs exhibited significant scavenging effects on DPPH and hydroxyl radicals,with IC50 values of 1.34 mg/mL and 1.42 mg/mL,respectively,and had a certain reducing power.Conclusion:These results suggested that flash extraction was an efficient and rapid method for obtaining AGFPs from AGF,and that AGFPs had promising antioxidant potential for further applications.This study can provide a theoretical basis for the development and application of AGF.
关键词
西洋参花多糖/闪式提取/工艺优化/抗氧化活性/响应面法Key words
American ginseng flower polysaccharides/flash extraction/process optimization/antioxidant activity/response surface methodology引用本文复制引用
基金项目
国家重点研发计划(2021YFD1600903)
吉林省大学生创新创业训练计划项目(S202210199030S)
出版年
2024
国家科技期刊平台
NSTL
万方数据