桑黄总黄酮的提取、组分分析及其生物活性评价
Extraction,Component Analysis and Biological Activity Evaluation of Total Flavonoids from Phellinus igniarius
付婷伟 1周紫微 1邵颖 2张朋 3宁晓雅 1杨洋 1陈安徽2
作者信息
- 1. 徐州工程学院食品(生物)工程学院,江苏徐州 221111
- 2. 徐州工程学院食品(生物)工程学院,江苏徐州 221111;江苏省食品资源开发与质量安全重点建设实验室,徐州工程学院,江苏徐州 221111
- 3. 徐州天益食品科技研究院有限公司,江苏徐州 210009
- 折叠
摘要
为系统研究桑黄中总黄酮的提取工艺,明晰总黄酮中黄酮类化合物的组成及含量,探讨桑黄总黄酮的生物活性,本研究采用响应面法优化了桑黄总黄酮的提取工艺,高效液相色谱法分析了桑黄总黄酮的组成,并检测其生物活性.实验结果表明,以桑黄总黄酮得率为指标的最优提取条件为提取温度 73℃、固液比 1:50 g/mL、提取时间 3 h,在此提取条件下桑黄总黄酮的得率达到了 2.68%;采用高效液相色谱法测定了桑黄总黄酮中的黄酮种类及其含量,发现桑黄总黄酮中主要含有花旗松素、槲皮素和山奈酚,其中花旗松素含量最高达到 3727.31 mg/kg;桑黄总黄酮生物活性分析结果显示,其具有一定的体外抗氧化、降脂、降糖活性,在其浓度为 14 μg/mL时对DPPH自由基的清除率达到 58.63%±0.45%,浓度为 0.08 mg/mL时,对羟基自由基的清除率达到 52.51%±1.49%;桑黄总黄酮的降脂活性实验结果显示,桑黄总黄酮在浓度为 6 mg/mL时对胰脂肪酶的抑制率达到 10.56%±0.06%,在浓度为 4 mg/mL时对胆固醇胶束溶解度的抑制率达到了 32.59%±0.78%;桑黄总黄酮具有明显的降糖活性,其对α-葡萄糖苷酶和α-淀粉酶的IC50 分别为71.42 mg/mL和97.28 mg/mL.
Abstract
In order to study the extraction technology of total flavonoids from Phellinus igniarius systematically,clarify the composition and content of flavonoids in total flavonoids,and explore the biological activity of total flavonoids from Phellinus igniarius.The extraction technology of Phellinus igniarius total flavonoids was optimized by response surface method,the composition of Phellinus igniarius total flavonoids was analyzed,and some of its biological activities were also detected.The results showed that the optimal extraction conditions were extraction temperature of 73℃,solid-liquid ratio of 1:50 g/mL and extraction time of 3 h,under which the yield of Phellinus igniarius total flavonoids reached 2.68%.The types and contents of flavonoids in Phellinus igniarius total flavonoids were determined by HPLC.Total flavonoids of Phellinus igniarius mainly contained taxifolin,quercetin and kaempferol,and the content of taxifolin was 3727.31 mg/kg.The analysis of biological activities of Phellinus igniarius total flavonoids showed that it had certain antioxidant,lipid-lowering and hypoglycemic activities in vitro,and the scavenging rate of DPPH free radicals reached 58.63%±0.45%when its concentration was 14 μg/mL,and the scavenging rate of hydroxyl free radicals reached 52.51%±1.49%when its concentration was 0.08 mg/mL.The experimental results of lipid-lowering activity showed that the inhibitory rate of Phellinus igniarius total flavonoids on pancreatic lipase reached 10.56%±0.06%at the concentration of 6 mg/mL,and the inhibitory rate on cholesterol micelle solubility reached 32.59%±0.78%at the concentration of 4 mg/mL.Phellinus igniarius total flavonids had obvious hypoglycemic activity,and its IC50 for α-glucosidase and α-amylase was 71.42 mg/mL and 97.28 mg/mL respectively.
关键词
桑黄/总黄酮/提取/组分分析/生物活性Key words
Phellinus igniarius/total foavonoids/extraction/component analysis/biological activity引用本文复制引用
基金项目
江苏省科技计划(XZ-SZ202150)
徐州市科技计划重点研发项目(KC20047)
出版年
2024
国家科技期刊平台
NSTL
万方数据