响应面法优化螺旋藻多糖提取及脱色工艺
Optimization of Extraction and Decolorization of Polysaccharides from Spirulina platensis by Response Surface Experiment
吴世林 1陈冉 1陈静芸 1杨宁 1李坤 1章真 1张荣庆2
作者信息
- 1. 上海海洋大学水产与生命学院,上海 201306;浙江清华长三角研究院浙江省应用酶学重点实验室,浙江嘉兴 314000
- 2. 浙江清华长三角研究院浙江省应用酶学重点实验室,浙江嘉兴 314000
- 折叠
摘要
研究螺旋藻多糖热碱液的提取、过氧化氢法脱色的条件并进行工艺优化.在单因素实验结果的基础上,分别以多糖得率和多糖脱色率为指标,通过响应面法优化热碱法提取螺旋藻多糖和过氧化氢法脱色的工艺条件,并分别得到热碱液提取螺旋藻多糖的最优提取工艺条件和过氧化氢法最优脱色条件.结果表明,热碱液提取法最优条件:NaOH质量分数 1.5%、提取温度为 90℃、提取时间 4 h及料液比 1:20 g/mL,在此条件下,得到螺旋藻多糖得率为 5.18%±0.23%;过氧化氢法的最佳脱色条件:过氧化氢溶液添加量为 17%、脱色时间为 55 min、脱色温度为 50℃,在此条件下得到的实际脱色率为 82.54%±0.03%,多糖保留率为 83.45%±0.13%.本研究所得结果对于螺旋藻多糖的开发利用具有一定参考价值.
Abstract
The extraction and decolorization conditions for Spirulina platensis polysaccharides using hot alkali and hydrogen peroxide were investigated,and the process was optimized.Based on the results of a single-factor test,the yield and decolorization rate of polysaccharides were taken as indices to optimize the technological conditions for extracting polysaccharides from Spirulina platensis using the hot alkali method and decolorizing them with hydrogen peroxide using the response surface methodology.The optimal conditions for hot alkali extraction of polysaccharides from Spirulina platensis were determined as follows:NaOH concentration of 1.5%,extraction temperature of 90℃,extraction time of 4 hours,and material-to-liquid ratio of 1:20 g/mL.Under these conditions,the yield of polysaccharides from Spirulina platensis was 5.18%±0.23%.The optimal conditions for decolorization using hydrogen peroxide were found to be:17%hydrogen peroxide solution,decolorization time of 55 minutes,and decolorization temperature of 50℃.Under these conditions,the actual decolorization rate was 82.54%±0.03%,and the retention rate of polysaccharides was 83.45%±0.13%.The findings of this study provide valuable insights for the development and utilization of Spirulina platensis polysaccharides.
关键词
螺旋藻/多糖/热碱法/提取/过氧化氢/脱色Key words
Spirulina platensis/polysaccharide/hot alkali extraction/extraction/hydrogen peroxide/decolorization引用本文复制引用
基金项目
浙江省基础公益研究计划项目(LGN22C190010)
出版年
2024
国家科技期刊平台
NSTL
万方数据