基于离子色谱-电感耦合等离子体质谱法分析木耳、香菇、松茸和茶树菇中砷形态分布
Analysis of Arsenic Speciation Distribution in Agaric,Shiitake Mushroom,Matsutake and Agrocybe by IC-ICP-MS Method
王小平 1刘忠莹 2钟洋 2张定秋 2陆阳 2朱敏敏 2郑红毅 2何叶馨 2王鑫 1黄韬睿 3江祖彬3
作者信息
- 1. 四川省食品检验研究院,四川成都 610097;国家市场监管重点实验室(白酒监管技术),四川成都 610097
- 2. 四川省食品检验研究院,四川成都 610097
- 3. 四川旅游学院,四川成都 610100
- 折叠
摘要
为分析木耳、香菇、松茸和茶树菇中砷形态分布,采用离子色谱-电感耦合等离子体质谱(Ion Chromato-graphy-Inductive Coupled Plasma Mass Spectrometer,IC-ICP-MS)法对其砷甜菜碱、二甲基砷、亚砷酸、砷胆碱、一甲基砷、砷酸进行测定,并进行方法学考察和含量测定.结果表明:方法在 5 min内能将 6种砷形态全部分离开,且峰型好;方法线性关系良好(标线质量浓度 0.5～20 μg/L,r＞0.999),6种砷形态检出限和定量限分别不超过 0.005、0.017 mg/kg.木耳、茶树菇和香菇中 6种砷形态加标回收率在 80%～120%范围;对于松茸,适宜加标量(0.05 mg/kg二甲基砷、砷胆碱和砷酸;0.2 mg/kg亚砷酸和一甲基砷;5 mg/kg砷甜菜碱),加标回收率也在80%～120%范围.结合干制品脱水率,所测样品无机砷含量都符合GB 2762-2022要求;松茸总砷含量最高,但是无机砷(亚砷酸+砷酸)占总砷比例最低为 3.7%～6.8%,其占比最高为砷甜菜碱(75.8%～87.3%);木耳、茶树菇和香菇中砷主要形式为无机砷,无机砷占总砷比例分别可达 58.4%～66.1%、60.0%～66.7%、81.2%～91.7%,提示其总砷高时有食用安全风险.
Abstract
To analyze the speciation distribution of arsenic in agaric,shiitake mushroom,matsutake and agrocybe,the ion chromatography-inductive coupled plasma mass spectrometer(IC-ICP-MS)was used to determine arsenobetaine,dimethyl arsenic,arsenous acid,arsenic choline,monomethyl arsenic and arsenic acid,and the methodological investigation and content determination were carried out.The results showed that the method could completely separate all six arsenic forms within 5 minutes,and the peak patterns were good.The linear relationship of the method was good(mass concentration of 0.5～20 μg/L,r＞0.999).The detection limit and quantification limit of six arsenic species were not more than 0.005 and 0.017 mg/kg respectively.The recovery rate of six arsenic forms in agaric,agrocybe and shiitake mushroom could reach 80%～120%with standard addition.For matsutake,the standard addition recovery rate could also reach 80%～120%when adding right standard amounts(0.05 mg/kg dimethyl arsenic,arsenic choline and arsenic acid;0.2 mg/kg arsenite and monomethyl arsenic;5 mg/kg arsenic betaine).Combined with the dehydration rate of dry products,the inorganic arsenic content of the tested samples met the requirements of GB 2762-2022.The content of total arsenic in matsutake was the highest,but the proportion of inorganic arsenic(arsenic choline+arsenic acid)in total arsenic was the lowest 3.6%～6.8%,and the highest proportion was arsenobetaine(75.8%～87.3%).The main form of arsenic in agaric,agrocybe,and shiitake mushroom were inorganic arsenic.The proportion of inorganic arsenic to total arsenic could reach 58.4%～66.1%、60.0%～66.7%、81.2%～91.7%,respectively.There was a risk of food safety when the total arsenic content was high.
关键词
木耳/香菇/松茸/茶树菇/离子色谱仪/电感耦合等离子体质谱仪/砷形态Key words
agaric/shiitake mushroom/matsutake/agrocybe/ion chromatograph/inductively coupled plasma mass spectrometer/arsenic speciation引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据