反复冻融法制备灵芝多糖工艺优化、结构表征及抗氧化活性分析
Repeated Freeze-thaw Extraction Optimization,Structural Features and Antioxidant Activity of Polysaccharides from Ganoderma lucidum
任洪飞 1逄梦玉 1隋昕怡 1刘丹 1杨飞燕 1刘养山 1张景 1杜秀菊1
作者信息
- 1. 聊城大学生命科学学院,山东聊城 252000
- 折叠
摘要
为提高灵芝多糖的提取得率,获得抗氧化活性更高的多糖组分,利用响应面试验优化反复冻融提取灵芝多糖的工艺条件,采用乙醇分级法对冻融多糖进行分离得到 3种多糖(GLPf30、GLPf60、GLPf80),评价热水浸提(G.lucidum polysaccharide water,GLPw)、反复冻融多糖(GLPf)及乙醇分级的多糖 GLPf30、GLPf60、GLPf80的抗氧化活性,以高效阴离子交换色谱(HPAEC)和红外光谱(FT-IR)进行结构表征.结果显示,最佳冻融条件为:溶胀比 14∶1(mL/g),冻融时间 140 min,冻融次数 3次.在此条件下,灵芝多糖提取得率为2.71%,明显高于传统水提(2.36%).HPAEC和FT-IR结果表明,5种多糖均是由六种单糖(岩藻糖、葡糖胺、半乳糖、葡萄糖、木糖和甘露糖)组成的以吡喃糖为骨架的酸性多糖,但它们的单糖组成和理化性质有较大差异,尤其是GLPf30的氨基葡萄糖(GlcN)占比最高(达到 37.66%).GLPf30的抗氧化能力明显高于其他两种多糖(GLPf60和GLPf80)及其前体物GLPf.研究结果为灵芝多糖的合理开发和高值化利用提供科学依据.
Abstract
A new repeated freeze-thaw extraction was employed to extract crude polysaccharide from Ganoderma lucidum,named as GLPf,in order to improve its extraction yield and bioactivity.Single-factor tests and response surface experiment were used to obtain the optimal extraction conditions.Three novel G.lucidum polysaccharide fractions(GLPf30,GLPf60,and GLPf80)were isolated and purified from GLPf by stepwise alcohol precipitation.Results showed that the optimal parameters were as follows:Swelling ratio 1∶14(g/mL),free-ze-thaw time 140 min,and free-ze-thaw times was 3.Under these conditions,its extraction rate was 2.71％,which was significantly higher than that of polysaccharide extrcted by traditional water extraction(GLP,2.36％).The preliminary structural features of all polysaccharide fractions were determined by highperformance anion-exchange chromatography with pulsed-amperometric detection(HPAEC-PAD)and Fourier transform infrared spectrophotometer(FT-IR)analyses,which showed that all sugar fractions were acidic polysaccharides,and they all were composed of six monosaccharides(fucose,glucosamine,galactose,glucose,xylose and mannose)with pyranose as the backbone.However,there were significant differences in both monosaccharide composition and physicochemical property,and GLPf30 had the highest percentage of GlcN(up to 37.66％).The results of antioxidant activity assays showed that antioxidant power of GLPf30 was significantly higher than that of the other two polysaccharides(GLPf60 and GLPf80)and their precursors GLPf.These findings would provide scientific basis for the rational development and high value utilization of G.lucidum polysaccharides.
关键词
灵芝多糖/反复冻融/工艺优化/抗氧化活性/结构表征Key words
Ganoderma lucidum polysaccharides/repeated freeze-thaw/process optimization/antioxidant activity/structural characterization引用本文复制引用
基金项目
山东省自然科学基金(ZR2015CL002)
聊城大学科研项目(318011707)
出版年
2024
国家科技期刊平台
NSTL
万方数据